An anther-specific gene PhGRP is regulated by PhMYC2 and causes male sterility when overexpressed in petunia anthers
An anther-specific gene PhGRP is regulated by PhMYC2 and causes male sterility when overexpressed in petunia anthers
复制标题
花药特异性基因 PhGRP 受 PhMYC2 调节，当在矮牵牛花药中过度表达时会导致雄性不育
DOI：
10.1007/s00299-017-2163-7
复制
发表时间：
2017-06
影响因子：
6.2
通讯作者：
Hu Huirong
中科院分区：
文献类型：
--
作者：
Yue Yuanzheng;Yin Chaoqun;Guo Rui;Peng Hao;Yang Zhaonan;Liu Guofeng;Bao Manzhu;Hu Huirong
An anther-specific GRP gene, regulated by PhMYC2 , causes a significant reduction of male fertility when overexpressed in petunia, and its promoter is efficient in genetic engineering of male-sterile lines. Glycine-rich proteins (GRPs) play important roles in plant anther development; however, the underlying mechanisms and related regulatory networks are poorly understood. In this study, a novel glycine-rich family gene designated as PhGRP was isolated from Petunia hybrida 'Fantasy Red'. The qRT-PCR analysis showed that it expressed specifically in anthers, and its expression peaked earlier than those well-known tapetum-specific genes, such as TA29, and several genes with the classic cis-regulatory element 'anther-box' in petunia during its anther development. The male fertility was significantly reduced in PhGRP overexpression lines, due to the abnormal formation of pollen wall. The PhGRP promoter (pPhGRP) could drive the GUS genes expressing specifically in the anthers of the transgenic Arabidopsis plants, indicating that the anther-specific characteristic of this promoter was conserved. In addition, when pPhGRP was used to drive the expression of BARNASE, complete male-sterile petunia lines were created without changes in vegetative organs and floral parts other than anthers. Finally, when pPhGRP was used as the bait to screen a yeast-one-hybrid (Y1H) library, a transcription factor (PhMYC2) belonging to the bHLH family was successfully selected, and the binding between pPhGRP and PhMYC2 was validated both by Y1H and dual-luciferase reporter assay. Overall, these results suggest that PhGRP, which is a male fertility-related gene that expresses specifically in anthers, is regulated by PhMYC2 and whose promoter can be used as an effective tool in the creation of male-sterile lines.
登录
查看更多内容
影响因子：
6.2
作者：
Long, Ruicai;Yang, Qingchuan;Zhang, Ze
通讯作者：
Zhang, Ze
影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
Sumner, Lloyd W.
影响因子：
7.4
作者：
Min, Ling;Li, Yaoyao;Zhang, Xianlong
通讯作者：
Zhang, Xianlong
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan
影响因子：
7.4
作者：
CONDIT, CM;MCLEAN, BG;MEAGHER, RB
通讯作者：
MEAGHER, RB