Cochlear motion across the reticular lamina implies that it is not a stiff plate.
Cochlear motion across the reticular lamina implies that it is not a stiff plate.
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DOI：
10.1038/s41598-022-23525-x
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发表时间：
2022-11-04
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
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在耳蜗内，基底膜(BM)通过三排压电样外毛细胞(OHC)和支持细胞与网状板(RL)相连，这些细胞赋予哺乳动物敏感的听力。OHC各行之间的解剖差异表明它们的运动方式不同。使用光学相干断层扫描，我们通过沙土鼠圆窗膜测量了大约40-47 GHz最佳频率(BF)区域的在体和死后位移。我们的高空间分辨率允许在RL表面的三排单独的HC及其底部的顶部进行测量，以及跨BM进行测量。RL运动呈径向变化；第三排的增益比第一排大3倍以上，而OHC-底部的运动保持不变。这意味着RL马赛克，由OHC和指突顶部通过黏附分子连接在一起，比Deiters的细胞在其底部表面连接到OHC要灵活得多。死后，测量点几乎同步移动在一起。这意味着在体内，RL并不像已经假设的那样作为一个僵硬的板在靠近第一排的柱状细胞头部周围运动，而是它的马赛克样结构可能会弯曲和/或拉伸。
Within the cochlea, the basilar membrane (BM) is coupled to the reticular lamina (RL) through three rows of piezo-like outer hair cells (OHCs) and supporting cells that endow mammals with sensitive hearing. Anatomical differences across OHC rows suggest differences in their motion. Using optical coherence tomography, we measured in vivo and postmortem displacements through the gerbil round-window membrane from approximately the 40–47 kHz best-frequency (BF) regions. Our high spatial resolution allowed measurements across the RL surface at the tops of the three rows of individual OHCs and their bottoms, and across the BM. RL motion varied radially; the third-row gain was more than 3 times greater than that of the first row near BF, whereas the OHC-bottom motions remained similar. This implies that the RL mosaic, comprised of OHC and phalangeal-process tops joined together by adhesion molecules, is much more flexible than the Deiters’ cells connected to the OHCs at their bottom surfaces. Postmortem, the measured points moved together approximately in phase. These imply that in vivo, the RL does not move as a stiff plate hinging around the pillar-cell heads near the first row as has been assumed, but that its mosaic-like structure may instead bend and/or stretch.
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