Multiparametric Sensing of Outer Membrane Vesicle-Derived Supported Lipid Bilayers Demonstrates the Specificity of Bacteriophage Interactions.
Multiparametric Sensing of Outer Membrane Vesicle-Derived Supported Lipid Bilayers Demonstrates the Specificity of Bacteriophage Interactions.
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DOI：
10.1021/acsbiomaterials.3c00021
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发表时间：
2023-06-12
影响因子：
5.8
通讯作者：
Owens, Roisin M.
中科院分区：
文献类型：
--
作者：
Bali, Karan;McCoy, Reece;Lu, Zixuan;Treiber, Jeremy;Savva, Achilleas;Kaminski, Clemens F.;Salmond, George;Salleo, Alberto;Mela, Ioanna;Monson, Rita;Owens, Roisin M.
关键词：
近年来，随着传统抗生素的有效性减弱，使用噬菌体作为抗生素已成为一个非常感兴趣的领域。快速、定量地检测噬菌体与特定细菌的相互作用是鉴定新型抗菌剂的关键。来自革兰氏阴性菌的外膜囊泡(OMV)可以用来制造支持的脂质双层(SLB)，因此可以在体外建立包含细菌外膜天然成分的膜模型。在这项研究中，我们使用了大肠杆菌OMV来源的SLB，并使用荧光成像和机械传感技术来显示它们与T4噬菌体的相互作用。我们还将这些双层膜与导电聚合物PEDOT：PSS功能化的微电极阵列(MEA)进行了集成，并表明可以用阻抗谱来监测噬菌体与SLB之间的成孔作用。为了突出我们检测特定噬菌体相互作用的能力，我们还使用来自轮齿柠檬酸杆菌的OMV生成SLB，该OMV对T4噬菌体感染具有抵抗力，并确定它们与噬菌体缺乏相互作用。这里介绍的工作展示了如何使用一系列实验技术来监测噬菌体和这些复杂的SLB系统之间发生的相互作用。我们相信这种方法可以用来识别对感兴趣的细菌菌株起作用的噬菌体，以及更广泛地监测与细菌外膜相互作用的任何孔形成结构(如防御素)，从而帮助开发下一代抗菌剂。
The use of bacteriophages, viruses that specifically infect bacteria, as antibiotics has become an area of great interest in recent years as the effectiveness of conventional antibiotics recedes. The detection of phage interactions with specific bacteria in a rapid and quantitative way is key for identifying phages of interest for novel antimicrobials. Outer membrane vesicles (OMVs) derived from Gram-negative bacteria can be used to make supported lipid bilayers (SLBs) and therefore in vitro membrane models that contain naturally occurring components of the bacterial outer membrane. In this study, we employed Escherichia coli OMV derived SLBs and use both fluorescent imaging and mechanical sensing techniques to show their interactions with T4 phage. We also integrate these bilayers with microelectrode arrays (MEAs) functionalized with the conducting polymer PEDOT:PSS and show that the pore forming interactions of the phages with the SLBs can be monitored using electrical impedance spectroscopy. To highlight our ability to detect specific phage interactions, we also generate SLBs using OMVs derived from Citrobacter rodentium, which is resistant to T4 phage infection, and identify their lack of interaction with the phage. The work presented here shows how interactions occurring between the phages and these complex SLB systems can be monitored using a range of experimental techniques. We believe this approach can be used to identify phages that work against bacterial strains of interest, as well as more generally to monitor any pore forming structure (such as defensins) interacting with bacterial outer membranes, and thus aid in the development of next generation antimicrobials.
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DOI：
10.1038/s41396-021-00921-1
发表时间：
2021-08
期刊：
The ISME journal
影响因子：
--
作者：
Carim S;Azadeh AL;Kazakov AE;Price MN;Walian PJ;Lui LM;Nielsen TN;Chakraborty R;Deutschbauer AM;Mutalik VK;Arkin AP
通讯作者：
Arkin AP
影响因子：
3.9
作者：
Bali, Karan;Mohamed, Zeinab;Scheeder, Anna;Pappa, Anna-Maria;Daniel, Susan;Kaminski, Clemens F.;Owens, Roisin M.;Mela, Ioanna
通讯作者：
Mela, Ioanna
影响因子：
9.5
作者：
Bali K;Guffick C;McCoy R;Lu Z;Kaminski CF;Mela I;Owens RM;van Veen HW
通讯作者：
van Veen HW
影响因子：
2.1
作者：
Choi, Sung-Eun;Greben, Kyrylo;Offenhaeusser, Andreas
通讯作者：
Offenhaeusser, Andreas
影响因子：
6.4
作者：
Behrens, Hannah M.;Lowe, Edward D.;Kleanthous, Colin
通讯作者：
Kleanthous, Colin