Application of High-Speed Counter-Current Chromatography Preparative Separation of Flavone C-Glycosides From Lophatherum gracile
Application of High-Speed Counter-Current Chromatography Preparative Separation of Flavone C-Glycosides From Lophatherum gracile
复制标题
应用高速逆流色谱制备淡竹叶黄酮C-苷
DOI：
10.1080/01496395.2012.762795
复制
发表时间：
2013-02
影响因子：
2.8
通讯作者：
Ye, Wencai
中科院分区：
文献类型：
--
作者：
Tang, Qingfa;Wang, Ying;Chen, Mei;Zhang, Qingwen;Fan, Chunlin;Huang, Xiaojun;Li, Yaolan;Ye, Wencai
采用制备型高速逆流色谱(HSCCC)分离纯化薄荷茎叶中的活性成分。采用优化的乙酸乙酯-正丁醇-乙醇-水两相体系，体积比为4：2：1.5：8.5(v/v)，经HSCCC一步分离得到6个黄酮类C-糖苷，纯度均在95%以上。木犀草素6-C-α-L-arabinopyranosyl-7-O-β-D-glucopyranoside(2)2 8.5 mg，异乌头菜素(3)31.5 mg，冬凌草素(4)44.8 mg，苦马豆素(5)2 5.3 mg，芹菜素6-C-β-D-半乳糖醛酸(1→2)-β-D-吡喃葡萄糖苷(6)。用高效液相色谱(HPLC法)测定化合物的纯度。其化学结构经电子喷雾电离质谱仪(ESI-MS)、核磁共振氢谱(1H)和核磁共振碳谱(13C)确证。
Preparative high-speed counter-current chromatography (HSCCC) was used to separate and purify bioactive constituents from the stems and leaves of Lophatherum gracile Brongn. Six flavone C-glycosides each at over 95% purity including two new compounds were obtained in one-step separation by HSCCC with an optimized two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water at volume ratio of 4:2:1.5:8.5 (v/v/v/v). The experiment yielded 19.9 mg of luteolin 6-C-β-D-galactopyranosiduronic acid (1→2)-β-D-glucopyranoside (1), 28.5 mg of luteolin 6-C-α-L-arabinopyranosyl-7-O-β-D-glucopyranoside (2), 31.5 mg of isoorientin (3), 44.8 mg of orientin (4), 25.3 mg of swertiajaponin (5) and 12.1 mg of apigenin 6-C-β-D-galactopyranosiduronic acid (1→2)-β-D-glucopyranoside (6) from 500 mg of crude extracts. The purity of these compounds was determined by high-performance liquid chromatography (HPLC). Their chemical structures were identified by electron spray ionization mass spectroscopy (ESI-MS), 1H and 13C nuclear magnetic resonance spectroscopy (NMR).
登录
查看更多内容
影响因子：
3.8
作者：
R. Rofi;A. Pomilio
通讯作者：
R. Rofi;A. Pomilio
DOI：
--
发表时间：
2011
期刊：
Journal of Anhui Agricultural University
影响因子：
--
作者：
Yong-de Yue
通讯作者：
Yong-de Yue
DOI：
10.1080/10826070903320756
发表时间：
2009-12-01
影响因子：
1.3
作者：
He D;Gu D;Huang Y;Ayupbek A;Yang Y;Aisa HA;Ito Y
通讯作者：
Ito Y
影响因子：
3.1
作者：
Haoyu Ye;Shijie Zhong;Yanfang Li;Ming-hai Tang;A. Peng;Jia Hu;Jie Shi;Shicao He;Wenshuang Wu;Li-juan Chen
通讯作者：
Haoyu Ye;Shijie Zhong;Yanfang Li;Ming-hai Tang;A. Peng;Jia Hu;Jie Shi;Shicao He;Wenshuang Wu;Li-juan Chen
影响因子：
3.1
作者：
Fanggui Ye;Baojun Huang;Shun Wang;Shulin Zhao
通讯作者：
Fanggui Ye;Baojun Huang;Shun Wang;Shulin Zhao