The Vaginal Microbiota Composition and Genital Infections during and after Pregnancy among Women in Pemba Island, Tanzania.
The Vaginal Microbiota Composition and Genital Infections during and after Pregnancy among Women in Pemba Island, Tanzania.
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DOI：
10.3390/microorganisms10030509
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发表时间：
2022-02-25
期刊：
影响因子：
4.5
通讯作者：
Ambrosino E
中科院分区：
文献类型：
--
作者：
Juliana NCA;Deb S;Juma MH;Poort L;Budding AE;Mbarouk A;Ali SM;Ouburg S;Morré SA;Sazawal S;Ambrosino E
我们调查了坦桑尼亚奔巴岛孕妇和产后妇女的阴道微生物区系(VMB)组成、生殖器病原体的流行率及其相关性。从90名妇女身上采集阴道拭子，在怀孕期间的两个时间点(胎龄20周[GA]和≥20周GA)和分娩后(如果可能)采集一次。采用IS-PRO法对VMB进行鉴定。用qPCRS检测沙眼衣原体(CT)、淋病奈瑟菌(NG)、阴道毛滴虫(TV)、生殖支原体(MG)和人乳头瘤病毒(HPV)。VMB孕期以乳酸菌为主，产后以非乳杆菌为主。在配对和未配对的样本中，在怀孕期间观察到VMB丰富度显著下降。在未配对的样本中，香农多样性在怀孕期间显著低于分娩后。克雷伯氏菌和链球菌是所有时间点最常见的致病菌。病原菌的大量存在主要见于患有非乳杆菌占优势的VMB的女性。在妊娠20周的≥时间点，携带一种或多种生殖器病原体(人乳头瘤病毒、CT、TV或MG)的妇女中，63.0%的妇女以乳杆菌为主。临产前未检出NG。这项研究为VMB的组成、孕期和产后的变化以及它们与病原体和生殖器病原体的关系提供了证据。
We investigated the vaginal microbiota (VMB) composition, prevalence of genital pathogens and their association among pregnant and post-delivery women in Pemba Island, Tanzania. Vaginal swabs were collected from 90 women, at two time points during pregnancy (<20 weeks of gestational age [GA] and ≥20 weeks GA) and once after delivery, when possible. IS-pro assay was used for VMB characterization. Chlamydia trachomatis (CT), Neisseria gonorrhea (NG), Trichomonas vaginalis (TV), Mycoplasma genitalium (MG) and human papillomavirus (HPV) were detected by qPCRs. VMB were mostly Lactobacillus dominant during pregnancy and non-Lactobacillus dominant post-delivery. A significant decrease in VMB richness was observed during pregnancy among paired and unpaired samples. Shannon diversity was significantly lower during pregnancy than post-delivery among unpaired samples. Klebsiella species and Streptococcus anginosus were the most commonly identified pathobionts at all timepoints. A high abundance of pathobionts was mostly seen in women with non-Lactobacillus dominant VMB. At ≥20 weeks GA timepoint during pregnancy, 63.0% of the women carrying one or more genital pathogen (either HPV, CT, TV, or MG) had L. iners dominant VMB. NG was not detected pre-delivery. This study contributes evidence on VMB composition, its changes during pregnancy and post-delivery, and their association with pathobionts and genital pathogens.
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影响因子：
9.3
作者：
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影响因子：
3.7
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影响因子：
4.6
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影响因子：
3.7
作者：
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通讯作者：
Versalovic J