Inactivation of Wolbachia Reveals Its Biological Roles in Whitefly Host
Inactivation of Wolbachia Reveals Its Biological Roles in Whitefly Host
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沃尔巴克氏体的灭活揭示了其在粉虱宿主中的生物学作用
DOI：
10.1371/journal.pone.0048148
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发表时间：
2012-10
期刊：
影响因子：
3.7
通讯作者：
Qiu, Bao-Li
中科院分区：
文献类型：
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作者：
Xue, Xia;Li, Shao-Jian;Ahmed, Muhammad Z.;De Barro, Paul J.;Ren, Shun-Xiang;Qiu, Bao-Li
背景烟粉虱是一个由众多种组成的隐种复合体。来自复合体的个体物种具有多种细菌内共生体，包括沃尔巴克氏体。然而，虽然已知沃尔巴克氏体有许多不同的作用，但它在B中的作用。烟粉虱不清楚。在这里，抗生素利福平用于选择性地从B中消除沃尔巴克氏体。烟粉虱，使其在粉虱发育和繁殖的作用进行了探讨。消除Wolbachia对B优势寄生蜂双斑恩蚜小蜂生物学的间接影响。烟粉虱在华南地区也进行了调查。方法学/主要发现qRT-PCR和FISH显示，暴露于1.0 mg/ml利福平48 h后，沃尔巴克氏体从B中完全失活。地中海烟粉虱（MED）没有任何显着的影响，无论是初级共生体，Portiera aleyrodidarum或任何其他次级内共生体存在。为了B。Wolbachia与烟粉虱MED的关系表明，Wolbachia与减少的幼虫发育时间、增加的幼虫完成发育的可能性、增加的成虫寿命和增加的雌性后代百分比有关。灭活与第4龄的身体大小的显着减少，这使我们推测沃尔巴克氏体是否可能有营养补充作用。若虫体型的缩小对其寄生蜂E.双斑类消除沃尔巴克氏体导致寄生卵的比例显着增加，完成了他们的发展，但减少的粉虱主机的大小也与一个显着减少的大小新兴的寄生成虫，这反过来又与一个显着减少成年寄生蜂寿命。结论/意义Wolbachia增加了适应性的粉虱宿主，并提供了一些保护，防止寄生。这些观察结果增加了我们对细菌内共生体所发挥作用的理解。
Background The whitefly Bemisia tabaci is cryptic species complex composed of numerous species. Individual species from the complex harbor a diversity of bacterial endosymbionts including Wolbachia. However, while Wolbachia is known to have a number of different roles, its role in B. tabaci is unclear. Here, the antibiotic rifampicin is used to selectively eliminate Wolbachia from B. tabaci so as to enable its roles in whitefly development and reproduction to be explored. The indirect effects of Wolbachia elimination on the biology of Encarsia bimaculata, a dominant parasitoid of B. tabaci in South China, were also investigated. Methodology/Principal Finding qRT-PCR and FISH were used to show that after 48 h exposure to 1.0 mg/ml rifampicin, Wolbachia was completely inactivated from B. tabaci Mediterranean (MED) without any significant impact on either the primary symbiont, Portiera aleyrodidarum or any of the other secondary endosymbionts present. For B. tabaci MED, Wolbachia was shown to be associated with decreased juvenile development time, increased likelihood that nymphs completed development, increased adult life span and increased percentage of female progeny. Inactivation was associated with a significant decrease in the body size of the 4th instar which leads us to speculate as to whether Wolbachia may have a nutrient supplementation role. The reduction in nymph body size has consequences for its parasitoid, E. bimaculata. The elimination of Wolbachia lead to a marked increase in the proportion of parasitoid eggs that completed their development, but the reduced size of the whitefly host was also associated with a significant reduction in the size of the emerging parasitoid adult and this was in turn associated with a marked reduction in adult parasitoid longevity. Conclusions/Significance Wolbachia increases the fitness of the whitefly host and provides some protection against parasitization. These observations add to our understanding of the roles played by bacterial endosymbionts.
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影响因子：
3.7
作者：
Chiel E;Zchori-Fein E;Inbar M;Gottlieb Y;Adachi-Hagimori T;Kelly SE;Asplen MK;Hunter MS
通讯作者：
Hunter MS
影响因子：
2
作者：
R. Dmochowski
通讯作者：
R. Dmochowski
DOI：
10.1016/j.respe.2020.09.004
发表时间：
2020-10-10
期刊：
Revue D'Epidemiologie et De Sante Publique
影响因子：
--
作者：
La rédaction
通讯作者：
La rédaction
影响因子：
9.3
作者：
通讯作者：
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