Amnion and chorion matrix maintain hMSC osteogenic response and enhance immunomodulatory and angiogenic potential in a mineralized collagen scaffold.
Amnion and chorion matrix maintain hMSC osteogenic response and enhance immunomodulatory and angiogenic potential in a mineralized collagen scaffold.
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DOI：
10.3389/fbioe.2022.1034701
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发表时间：
2022
影响因子：
5.7
通讯作者：
Harley, Brendan A. C.
中科院分区：
文献类型：
--
作者：
Kolliopoulos, Vasiliki;Dewey, Marley J.;Polanek, Maxwell;Xu, Hui;Harley, Brendan A. C.
颅颌面（CMF）骨损伤是一项重大的外科挑战，由于其尺寸大且地形复杂，无法自然愈合。我们正在开发一种矿化胶原蛋白支架，它模拟骨的细胞外基质（ECM）特征。这些支架在体外可诱导人间充质干细胞（hMSC）成骨分化，在体内可促进骨形成，且无需外源性成骨补充剂。在此，我们试图通过在支架结构中加入胎盘衍生产品来增强促再生潜力。羊膜和绒毛膜是胎盘的不同组成部分，它们各自都显示出抗炎、免疫调节和成骨特性。虽然这对我们的矿化胶原蛋白支架可能是一种有力的改进，但加入的途径（基质固定或可溶性）尚未得到很好的理解。在此我们比较了将羊膜和绒毛膜基质以及从这些膜中提取的可溶性提取物引入胶原蛋白支架对支架生物物理特性以及由此产生的hMSC成骨活性的影响。虽然在制造过程中将羊膜和绒毛膜基质纳入支架微结构不会影响其孔隙率，但会影响压缩性能。在制造后将羊膜的可溶性提取物纳入支架，与传统的矿化胶原蛋白支架相比，可诱导hMSC产生最高水平的代谢活性以及相当的矿物质沉积和破骨细胞抑制剂骨保护素（OPG）的洗脱。矿化胶原蛋白 - 羊膜复合支架在hMSCs中引发了增强的早期成骨基因表达（BGLAP，BMP2），增加了免疫调节基因表达（CCL2，HGF和MCSF）以及增加了血管生成基因表达（ANGPT1，VEGFA）。矿化胶原蛋白 - 绒毛膜复合支架促进了hMSCs中的免疫调节基因表达（CCL2，HGF和IL6），而不影响成骨基因表达。总之，这些发现表明，使用来自羊膜和绒毛膜的基质改性的矿化胶原蛋白支架代表了一种有利于颅颌面骨修复的有前景的环境。
Craniomaxillofacial (CMF) bone injuries present a major surgical challenge and cannot heal naturally due to their large size and complex topography. We are developing a mineralized collagen scaffold that mimics extracellular matrix (ECM) features of bone. These scaffolds induce in vitro human mesenchymal stem cell (hMSC) osteogenic differentiation and in vivo bone formation without the need for exogenous osteogenic supplements. Here, we seek to enhance pro-regenerative potential via inclusion of placental-derived products in the scaffold architecture. The amnion and chorion membranes are distinct components of the placenta that each have displayed anti-inflammatory, immunomodulatory, and osteogenic properties. While potentially a powerful modification to our mineralized collagen scaffolds, the route of inclusion (matrix-immobilized or soluble) is not well understood. Here we compare the effect of introducing amnion and chorion membrane matrix versus soluble extracts derived from these membranes into the collagen scaffolds on scaffold biophysical features and resultant hMSC osteogenic activity. While inclusion of amnion and chorion matrix into the scaffold microarchitecture during fabrication does not influence their porosity, it does influence compression properties. Incorporating soluble extracts from the amnion membrane into the scaffold post-fabrication induces the highest levels of hMSC metabolic activity and equivalent mineral deposition and elution of the osteoclast inhibitor osteoprotegerin (OPG) compared to the conventional mineralized collagen scaffolds. Mineralized collagen-amnion composite scaffolds elicited enhanced early stage osteogenic gene expression (BGLAP, BMP2), increased immunomodulatory gene expression (CCL2, HGF, and MCSF) and increased angiogenic gene expression (ANGPT1, VEGFA) in hMSCs. Mineralized collagen-chorion composite scaffolds promoted immunomodulatory gene expression in hMSCs (CCL2, HGF, and IL6) while unaffecting osteogenic gene expression. Together, these findings suggest that mineralized collagen scaffolds modified using matrix derived from amnion and chorion membranes represent a promising environment conducive to craniomaxillofacial bone repair.
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影响因子：
3.4
作者：
Dewey, Marley J.;Kolliopoulos, Vasiliki;Harley, Brendan A. C.
通讯作者：
Harley, Brendan A. C.
影响因子：
3.5
作者：
Katagiri W;Takeuchi R;Saito N;Suda D;Kobayashi T
通讯作者：
Kobayashi T
影响因子：
5.8
作者：
Hortensius, Rebecca A.;Ebens, Jill H.;Harley, Brendan A. C.
通讯作者：
Harley, Brendan A. C.
DOI：
10.1186/2047-1440-1-12
发表时间：
2012-09-28
期刊：
Transplantation research
影响因子：
--
作者：
Eggenhofer E;Hoogduijn MJ
通讯作者：
Hoogduijn MJ
影响因子：
5.2
作者：
Bailey S;Karsenty G;Gundberg C;Vashishth D
通讯作者：
Vashishth D