3D-Printed Soft Membrane for Periodontal Guided Tissue Regeneration.
3D-Printed Soft Membrane for Periodontal Guided Tissue Regeneration.
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DOI：
10.3390/ma16041364
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发表时间：
2023-02-06
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--
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中科院分区：
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目的：目前的研究旨在使用临界尺寸的牙周犬模型进行体内检查，以研究3d打印软膜用于引导组织再生（GTR）的能力。这种膜是由明胶、弹性蛋白和透明质酸钠的特定组合物制成的，在我们之前的研究中对其进行了微调并充分表征。这种组合物的价值在于它有可能被用作胶原蛋白的合适替代品，胶原蛋白是传统GTR膜的主要成分，以克服胶原蛋白的成本问题。方法：采用手术方法在双侧犬牙根颊面形成临界尺寸的裂孔缺损。使用3d打印膜和两种市售胶原膜（Botiss Jason®和smartmembrane - regedent膜）进行GTR处理，未放置任何膜的组作为对照组。缺损用无张力牙龈瓣闭合淹没。在8周的实验期内，采用组织学和组织计量学分析评估牙周愈合情况。结果：组织计量学评估证实3d打印膜组新骨形成水平较高。8周后观察到所有缺损的牙周组织、骨、牙周韧带、牙骨质的形成，而对照组缺损部位仅出现结缔组织。在任何一组中都没有出现炎症或牙龈萎缩的临床症状。意义：3d打印明胶/弹性蛋白/透明质酸钠膜可以安全有效地用于牙周组织再生治疗的GTR，其效果优于或可与商业胶原膜相媲美。
Objectives: The current study aimed to perform an in vivo examination using a critical-size periodontal canine model to investigate the capability of a 3D-printed soft membrane for guided tissue regeneration (GTR). This membrane is made of a specific composition of gelatin, elastin, and sodium hyaluronate that was fine-tuned and fully characterized in vitro in our previous study. The value of this composition is its potential to be employed as a suitable replacement for collagen, which is the main component of conventional GTR membranes, to overcome the cost issue with collagen. Methods: Critical-size dehiscence defects were surgically created on the buccal surface of the roots of canine bilateral mandibular teeth. GTR treatment was performed with the 3D-printed membrane and two commercially available collagen membranes (Botiss Jason® and Smartbrane-Regedent membranes) and a group without any membrane placement was considered as the control group. The defects were submerged with tension-free closure of the gingival flaps. Histologic and histometric analyses were employed to assess the periodontal healing over an 8-week experimental period. Results: Histometric evaluations confirmed higher levels of new bone formation in the 3D-printed membrane group. Moreover, in all defects treated with the membranes, the formation of periodontal tissues, bone, periodontal ligaments, and cementum was observed after 8 weeks, while in the control group, only connective tissue was found in the defect sites. There was no clinical sign of inflammation or recession of gingiva in any of the groups. Significance: The 3D-printed gelatin/elastin/sodium hyaluronate membrane can be safe and effective for use in GTR for periodontal tissue regeneration therapies, with better or comparable results to the commercial collagen membranes.
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