Parallel Recording of Single Ion Channels: A Heterogeneous System Approach
Parallel Recording of Single Ion Channels: A Heterogeneous System Approach
复制标题
单离子通道的并行记录：异质系统方法
DOI：
--
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发表时间：
2010
影响因子：
2.4
通讯作者：
M. Tartagni
中科院分区：
文献类型：
--
作者：
F. Thei;M. Rossi;M. Bennati;M. Crescentini;F. Lodesani;H. Morgan;M. Tartagni
集成电子器件与纳米技术结构在异构系统上的融合为开发新型快速，灵敏和可靠的传感器提供了很好的机会。用传感元件嵌入微电子读出结构的主要优点是双重的。一方面，SNR由于缩放而增加。另一方面，读出微型化允许将传感器组织成阵列。后一点将通过使用统计方法来提高感测精度。然而，需要精确的接口设计来建立基于离子的信号和基于电子的信号之间的有效通信。本文展示了第一个例子的并发读出系统与单离子通道分辨率，使用紧凑和可扩展的架构。生物纳米传感器阵列被组织在不同的层上，以混合结构堆叠在一起：流体，印刷电路板和微电子读出。更具体地说，将与超低噪声Σ-Δ转换器电流放大器耦合的聚甲醛均聚物（POMH或Delrin）装置中加工的微孔阵列用于形成其中嵌入离子通道的双层膜。它示出了如何形成多个人工双层类脂膜（BLMs）的接口自动监测。该系统用于检测PA范围内的电流信号，来自单个BLM嵌入的μ-溶血素孔和β-环糊精分子之间的非共价结合。电流信号由读出结构同时处理。
The convergence of integrated electronic devices with nanotechnology structures on heterogeneous systems presents promising opportunities for the development of new classes of rapid, sensitive, and reliable sensors. The main advantage of embedding microelectronic readout structures with sensing elements is twofold. On the one hand, the SNR is increased as a result of scaling. On the other, readout miniaturization allows organization of sensors into arrays. The latter point will improve sensing accuracy by using statistical methods. However, accurate interface design is required to establish efficient communication between ionic-based and electronic-based signals. This paper shows a first example of a concurrent readout system with single-ion channel resolution, using a compact and scalable architecture. An array of biological nanosensors is organized on different layers stacked together in a mixed structure: fluidics, printed circuit board, and microelectronic readout. More specifically, an array of microholes machined into a polyoxymethylene homopolymer (POMH or Delrin) device coupled with ultralow noise sigma-delta converters current amplifiers, is used to form bilayer membranes within which ion channels are embedded. It is shown how formation of multiple artificial bilayer lipid membranes (BLMs) is automatically monitored by the interface. The system is used to detect current signals in the pA range, from noncovalent binding between single, BLM-embedded ¿-hemolysin pores and ß-cyclodextrin molecules. The current signals are concurrently processed by the readout structure.
影响因子：
4
作者：
Fologea, Daniel;Ledden, Bradley;Li, Jiali
通讯作者：
Li, Jiali