Disordered oropharyngeal microbial communities in H7N9 patients with or without secondary bacterial lung infection.
Disordered oropharyngeal microbial communities in H7N9 patients with or without secondary bacterial lung infection.
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伴或不伴继发细菌性肺部感染的 H7N9 患者口咽部微生物群落紊乱
DOI：
10.1038/emi.2017.101
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发表时间：
2017-12-20
影响因子：
13.2
通讯作者：
Li LJ
中科院分区：
文献类型：
--
作者：
Lu HF;Li A;Zhang T;Ren ZG;He KX;Zhang H;Yang JZ;Luo QX;Zhou K;Chen CL;Chen XL;Wu ZW;Li LJ
继发性细菌性肺部感染（SBLI）是H7N9病毒感染患者的严重并发症，并增加疾病的严重程度。口咽（OP）微生物组有助于防止呼吸道病原体的定植。我们的目的是使用16 S rRNA基因测序来研究伴/不伴继发性细菌性肺炎的H7 N9患者的OP微生物组。从51名H7N9患者（21名患有SBLI，30名没有）和30名匹配的健康对照（HC）中收集OP拭子样本，并用于微生物群落的比较组成，多样性和丰富度分析。主坐标分析成功区分了H7N9患者和健康受试者的OP微生物组，SBLI患者的OP微生物组多样性显著增加。与HC相比，H7N9患者的OP微生物组存在显著的生态失调，SBLI患者中有丰富的Leptotrichia，Oribacterium，Streptococcus，Atopobium，Eubacterium，Solobacterium和Rothiaspecies，而无SBLI患者中有Filifactor，Megasphaera和Leptotrichiaspecies。重要的是，嗜血杆菌和拟杆菌物种富集在HC中。这些发现揭示了H7N9患者OP微生物群的生态失调，并确定了SBLI的OP微生物风险指标，表明OP微生物群可以为SBLI预防的早期微生物群靶向预防性治疗提供新的非侵入性诊断生物标志物。Emerging Microbes & Infections（2017）6，e112; doi：10.1038/emi.2017.101;在线发表2017年12月20日
Secondary bacterial lung infection (SBLI) is a serious complication in patients with H7N9 virus infection, and increases disease severity. The oropharyngeal (OP) microbiome helps prevent colonisation of respiratory pathogens. We aimed to investigate the OP microbiome of H7N9 patients with/without secondary bacterial pneumonia using 16S rRNA gene sequencing. OP swab samples were collected from 51 H7N9 patients (21 with SBLI and 30 without) and 30 matched healthy controls (HCs) and used for comparative composition, diversity and richness analyses of microbial communities. Principal coordinates analysis successfully distinguished between the OP microbiomes of H7N9 patients and healthy subjects, and the OP microbiome diversity of patients with SBLI was significantly increased. There was significant dysbiosis of the OP microbiome in H7N9 patients, with an abundance ofLeptotrichia,Oribacterium,Streptococcus,Atopobium,Eubacterium,SolobacteriumandRothiaspecies in patients with SBLI, andFilifactor,MegasphaeraandLeptotrichiaspecies in patients without SBLI, when compared with HCs. Importantly,HaemophilusandBacteroidesspecies were enriched in HCs. These findings revealed dysbiosis of the OP microbiota in H7N9 patients, and identified OP microbial risk indicators of SBLI, suggesting that the OP microbiome could provide novel and non-invasive diagnostic biomarkers for early microbiota-targeted prophylactic therapies for SBLI prevention.Emerging Microbes & Infections(2017)6,e112; doi:10.1038/emi.2017.101; published online 20 December 2017
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影响因子：
6.4
作者：
Laufer AS;Metlay JP;Gent JF;Fennie KP;Kong Y;Pettigrew MM
通讯作者：
Pettigrew MM
影响因子：
2.7
作者：
Castro-Nallar E;Bendall ML;Pérez-Losada M;Sabuncyan S;Severance EG;Dickerson FB;Schroeder JR;Yolken RH;Crandall KA
通讯作者：
Crandall KA
人类感染新型禽源甲型流感 (H7N9) 病毒。
影响因子：
158.5
作者：
Gao, Rongbao;Cao, Bin;Shu, Yuelong
通讯作者：
Shu, Yuelong
DOI：
10.1073/pnas.1019378108
发表时间：
2011-03-29
影响因子：
11.1
作者：
Ichinohe, Takeshi;Pang, Iris K.;Iwasaki, Akiko
通讯作者：
Iwasaki, Akiko
影响因子：
11
作者：
Degnan, Patrick H.;Ochman, Howard
通讯作者：
Ochman, Howard