Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.
Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.
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DOI：
10.1126/sciadv.adg3433
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发表时间：
2023-05-10
期刊：
影响因子：
13.6
通讯作者：
Cristea, Ileana M.
中科院分区：
文献类型：
--
作者：
Song, Bokai;Sheng, Xinlei;Justice, Joshua L.;Lum, Krystal K.;Metzger, Peter J.;Cook, Katelyn C.;Kostas, James C.;Cristea, Ileana M.
感染细胞与周围组织细胞之间的通讯是病毒传播的决定因素。然而，目前尚不清楚与受感染细胞接近或远离的细胞如何对原发性或继发性感染作出反应。我们建立了一个基于细胞的系统来表征病毒微环境，区分感染的，邻近的和远端的细胞。细胞分选、显微镜、蛋白质组学和细胞周期测定允许解析接近感染的细胞特征和功能后果。我们发现，人巨细胞病毒（HCMV）感染引发了邻近细胞随后的HCMV感染和单纯疱疹病毒1型和A型流感的继发感染。邻近细胞表现出有丝分裂停滞、先天免疫力减弱和细胞外基质改变。相反，由于增强的抗病毒反应，远端细胞准备减缓病毒传播。这些发现表明感染如何通过细胞间信号重塑微环境以促进传播，以及空间接近感染如何引导细胞命运。病毒微环境中的细胞空间地址驱动蛋白质组，细胞周期和感染易感性的签名。
Communication between infected cells and cells in the surrounding tissue is a determinant of viral spread. However, it remains unclear how cells in close or distant proximity to an infected cell respond to primary or secondary infections. We establish a cell-based system to characterize a virus microenvironment, distinguishing infected, neighboring, and distal cells. Cell sorting, microscopy, proteomics, and cell cycle assays allow resolving cellular features and functional consequences of proximity to infection. We show that human cytomegalovirus (HCMV) infection primes neighboring cells for both subsequent HCMV infections and secondary infections with herpes simplex virus 1 and influenza A. Neighboring cells exhibit mitotic arrest, dampened innate immunity, and altered extracellular matrix. Conversely, distal cells are poised to slow viral spread due to enhanced antiviral responses. These findings demonstrate how infection reshapes the microenvironment through intercellular signaling to facilitate spread and how spatial proximity to an infection guides cell fate. Cell spatial address in a virus microenvironment drives signatures in proteomes, cell cycle, and susceptibility to infection.
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影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.1083/jcb.201608128
发表时间：
2017-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hua R;Cheng D;Coyaud É;Freeman S;Di Pietro E;Wang Y;Vissa A;Yip CM;Fairn GD;Braverman N;Brumell JH;Trimble WS;Raught B;Kim PK
通讯作者：
Kim PK
DOI：
10.1080/10409238.2016.1226250
发表时间：
2016
影响因子：
6.5
作者：
Crow MS;Lum KK;Sheng X;Song B;Cristea IM
通讯作者：
Cristea IM
影响因子：
6.7
作者：
Hertel L;Chou S;Mocarski ES
通讯作者：
Mocarski ES
影响因子：
6.7
作者：
Eifler M;Uecker R;Weisbach H;Bogdanow B;Richter E;König L;Vetter B;Lenac-Rovis T;Jonjic S;Neitzel H;Hagemeier C;Wiebusch L
通讯作者：
Wiebusch L