siRNA design principles and off-target effects.
siRNA design principles and off-target effects.
复制标题
siRNA设计原理和脱靶效应。
作者：
Sebastian Petri;G. Meister
短干扰rna （sirna）是一种主要的研究工具，它允许在几乎所有真核生物中通过选择性mRNA破坏来敲除靶基因。sirna通常由合成的~ 21个核苷酸（nt） rna双链组成，其中一条链与目标mRNA具有完美的互补性。尽管siRNA最初被认为是非常靶向的，因为它们的设计，但在过去的几年里，事实证明，所有siRNA都或多或少地具有明显的内在脱靶活性，这使得siRNA实验数据的解释变得困难。在这里，我们描述了siRNA设计的基本规则，为了获得具有最小脱靶活性的有效siRNA，应该考虑这些规则。此外，我们描述了如何在siRNA实验中控制脱靶活性。
Short interfering RNAs (siRNAs) are a major research tool that allows for knock-down of target genes via selective mRNA destruction in almost all eukaryotic organisms. siRNAs typically consist of a synthetic ∼21 nucleotide (nt) RNA-duplex where one strand is designed with perfect complementarity to the target mRNA. Although siRNAs were initially thought to be very target-specific because of their design, it turned out during the last years that all siRNAs have a more or less pronounced intrinsic off-target activity which can make the interpretation of data from siRNA experiments difficult. Here we describe essential rules for siRNA design that should be taken into account in order to obtain potent siRNAs with minimal off-target activity. In addition, we describe how to control for off-target activity in siRNA experiments.
影响因子：
4.5
作者：
Petri, Sebastian;Dueck, Anne;Meister, Gunter
通讯作者：
Meister, Gunter
影响因子：
10.5
作者：
Doench, JG;Petersen, CP;Sharp, PA
通讯作者：
Sharp, PA