Cholesterol depletion reduces apical transport capacity in epithelial Madin-Darby canine kidney cells.
Cholesterol depletion reduces apical transport capacity in epithelial Madin-Darby canine kidney cells.
复制标题
胆固醇消耗降低了上皮 Madin-Darby 犬肾细胞的顶端转运能力。
DOI：
10.1042/bj3570011
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Simons
中科院分区：
文献类型：
--
作者：
K. Prydz;K. Simons
降低Madin-Darby犬肾上皮（MDCK）细胞膜中的胆固醇水平，逆转顶端膜标志蛋白流感病毒血凝素和分泌的糖蛋白gp 80的顶端至基底外侧转运比。同时，水泡性口炎病毒G蛋白的基底外侧转运不受影响[Keller和Simons（1998）J. Cell Biol.140，1357-1367]。为了研究胆固醇耗竭是否会影响顶端分选机制，特别是顶端运输能力更普遍，我们研究了胆固醇耗竭对MDCK细胞层的顶端和基底侧表面的三种不同类型的分子分泌的影响：糖蛋白gp 80，硫酸化蛋白多糖和蛋白质，和非糖基化大鼠生长激素。在每种情况下，胆固醇消耗减少分泌到顶端培养基的分数，并增加分泌的分数。事实上，这是观察到的所有硫酸化的蛋白质和蛋白聚糖和非糖基化的大鼠生长激素，这是随机分泌在未经处理的细胞，表明胆固醇消耗减少顶端运输能力，而不是干扰特定的识别和分选过程。
Reduction of the cholesterol level in membranes of epithelial Madin-Darby canine kidney (MDCK) cells reverses the apical-to-basolateral transport ratio of the apical membrane marker protein influenza virus haemagglutinin and the secreted glycoprotein gp80. At the same time, basolateral transport of the vesicular stomatitis virus G protein is unaffected [Keller and Simons (1998) J. Cell Biol. 140, 1357-1367]. To investigate whether cholesterol depletion influences apical sorting mechanisms specifically, or apical transport capacity more generally, we studied the effect of cholesterol depletion on the secretion of three different classes of molecules from the apical and basolateral surfaces of MDCK cell layers: glycoprotein gp80, sulphated proteoglycans and proteins, and non-glycosylated rat growth hormone. In each case, cholesterol depletion reduced the fraction secreted to the apical medium and increased the fraction secreted basolaterally. The fact that this was observed for all sulphated proteins and proteoglycans and for the non-glycosylated rat growth hormone, which is randomly secreted in untreated cells, indicates that cholesterol depletion reduces the apical transport capacity, rather than interfering with specific recognition and sorting processes.
Thy-1 在上皮细胞中表达两种顶端定位信号。
DOI：
10.1152/ajpcell.1991.260.4.c715
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Powell,SK;Lisanti,MP;Rodriguez-Boulan,EJ
通讯作者：
Rodriguez-Boulan,EJ
影响因子：
19
作者：
E. Rodriguez-Boulan;Alfonso González
通讯作者：
E. Rodriguez-Boulan;Alfonso González
DOI：
10.1073/pnas.97.7.3248
发表时间：
2000
影响因子：
11.1
作者：
Marmorstein,AD;Csaky,KG;Baffi,J;Lam,L;Rahaal,F;Rodriguez-Boulan,E
通讯作者：
Rodriguez-Boulan,E