Transcriptomic, Biochemical, and Morphological Study Reveals the Mechanism of Inhibition of Pseudopestalotiopsis camelliae-sinensis by Phenazine-1-Carboxylic Acid.
Transcriptomic, Biochemical, and Morphological Study Reveals the Mechanism of Inhibition of Pseudopestalotiopsis camelliae-sinensis by Phenazine-1-Carboxylic Acid.
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转录组学、生化和形态学研究揭示吩嗪-1-羧酸抑制茶拟多盘病的机制
DOI：
10.3389/fmicb.2021.618476
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发表时间：
2021
影响因子：
5.2
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Yin Q;Yang R;Ren Y;Yang Z;Li T;Huang H;Tang Q;Li D;Jiang S;Wu X;Wang D;Chen Z
茶树灰枯病是茶树上危害最大的病害之一，在世界茶区广泛发生。该病由多种真菌植物病原菌引起，其中茶树假单胞菌是中国的主要病原菌。环境友好的抗菌剂吩嗪-1-羧酸(PCA)是一种天然土传细菌假单胞菌的代谢产物，可以抑制一系列真菌作物病害。在这项研究中，我们确定了PCA对Ps具有活性。山茶花的体外培养。我们用显微观察、转录、生化方法和分子对接等方法研究了PCA对菌丝的作用方式。扫描和透射电子显微镜观察结果表明，PCA可引起菌丝发育畸形和细胞器损伤，显著减少菌丝表面胞外多糖的积累。转录组分析表明，1705和1683两个基因存在差异表达。经PCA处理的茶树分别上调或下调，其中与核糖体生物发生、氧化磷酸化和N-糖链生物合成的各种蛋白编码相关的基因显著上调。上调与Ps生物合成N-糖链相关的9个基因。经逆转录定量聚合酶链式反应证实了茶树对PCA处理的反应。PCA处理显著降低了菌丝过氧化氢酶和超氧化物歧化酶的活性。我们的结果表明，暴露于PCA会导致氧化还原酶基因表达的变化，活性氧的积累，过氧化氢酶活性的降低，并伴随着对真菌细胞膜和细胞壁的损伤。
Gray blight disease is one of the most destructive diseases of tea plants and occurs widely in the tea-growing areas of the world. It is caused by several fungal phytopathogens, of which Pseudopestalotiopsis camelliae-sinensis is the main pathogen in China. The environmentally friendly antimicrobial, phenazine-1-carboxylic acid (PCA), a metabolite of the natural soil-borne bacteria Pseudomonas spp., can inhibit a range of fungal crop diseases. In this study, we determined that PCA was active against Ps. camelliae-sinensis in vitro. We studied the mode of action of PCA on hyphae using a microscopic investigation, transcriptomics, biochemical methods, and molecular docking. The results of scanning and transmission electron microscopy indicated that PCA caused developmental deformity of mycelia and organelle damage, and it significantly decreased the accumulation of exopolysaccharides on the hyphal surface. The transcriptome revealed that 1705 and 1683 differentially expressed genes of Ps. camelliae-sinensis treated with PCA were up-regulated or down-regulated, respectively, with genes associated with ribosome biogenesis, oxidative phosphorylation, and encoding various proteins of N-glycan biosynthesis being significantly up-regulated. Up-regulation of nine genes related to N-glycan biosynthesis of Ps. camelliae-sinensis in response to PCA treatment was confirmed by reverse transcription qPCR. The enzymatic activity of catalase and superoxide dismutase of hyphae was significantly decreased by PCA treatment. Our results indicated that exposure to PCA resulted in expression changes in oxidoreductase genes, accumulation of reactive oxygen species, and decreased activity of catalase, with concomitant damage to the fungal cell membrane and cell wall.
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影响因子：
6.3
作者：
Genheden S;Ryde U
通讯作者：
Ryde U
山茶花多拟盘拟盘属及近缘属，附中国11个新种的描述
影响因子：
4.6
作者：
Liu F;Hou L;Raza M;Cai L
通讯作者：
Cai L
影响因子：
48
作者：
Mortazavi, Ali;Williams, Brian A.;Wold, Barbara
通讯作者：
Wold, Barbara
影响因子：
48
作者：
Kim D;Langmead B;Salzberg SL
通讯作者：
Salzberg SL
影响因子：
3
作者：
Camacho C;Coulouris G;Avagyan V;Ma N;Papadopoulos J;Bealer K;Madden TL
通讯作者：
Madden TL