Monocyte Chemoattractant Protein-1 and Interleukin-8 Levels in Urine and Serum of Patents with Hemolytic Uremic Syndrome
Monocyte Chemoattractant Protein-1 and Interleukin-8 Levels in Urine and Serum of Patents with Hemolytic Uremic Syndrome
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溶血性尿毒症综合征患者尿液和血清中单核细胞趋化蛋白 1 和白细胞介素 8 水平
作者：
P. V. Setten;V. Hinsbergh;L. Heuvel;F. Preyers;H. Dijkman;K. Assmann;T. Velden;L. Monnens
儿童溶血性尿毒症综合征（HUS）的流行形式以内皮细胞损伤为特征，主要表现在肾小球毛细血管。单核细胞（MO）和多形核细胞（pmn）流入肾小球可能是溶血性尿毒综合征患者肾小球内皮细胞损伤开始、延长和进展的一个重要事件。这些白细胞进入肾脏募集的分子机制尚不清楚，但单核细胞趋化蛋白-1 （MCP-1）和IL-8被认为是主要的候选者。在这项研究中，我们通过特异性elisa分析了溶血性尿毒综合征儿童24小时尿液（n = 15）和血清（n = 14）样本中这两种趋化因子的存在。此外，我们用组织化学技术和电子显微镜检查了三名不同溶血性尿毒综合征儿童的肾脏活检，以检查MO和PMN细胞的浸润情况。虽然趋化因子MCP-1和IL-8在17名正常对照受试者的尿液中只存在非常有限的量，但溶血性尿毒综合征患者的一系列样本显示这两种趋化因子的水平显著升高。无尿的溶血性尿毒综合征患儿的初始和最大趋化因子水平高于无尿的患儿。尿MCP-1与IL-8水平呈显著正相关。溶血性尿毒综合征患儿血清IL-8水平显著升高，而与对照组相比，MCP-1水平仅略有升高。尿和血清趋化因子浓度之间没有相关性。溶血性尿毒症活检标本的组织学和EM研究清楚地显示肾小球中存在MOs和较小程度的pmn。目前的数据表明，在肾小球内皮细胞损伤过程中，MOs和pmn具有重要的局部作用。趋化因子MCP-1和IL-8可能分别通过募集和激活MOs和pmn参与溶血性毒血症的发病机制。
The epidemic form of the hemolytic uremic syndrome (HUS) in children is hallmarked by endothelial cell damage, most predominantly displayed by the glomerular capillaries. The influx of mononuclear (MO) and polymorphonuclear cells (PMNs) into the glomeruli may be an important event in the initiation, prolongation, and progression of glomerular endothelial cell damage in HUS patients. The molecular mechanisms for the recruitment of these leukocytes into the kidney are unclear, but monocyte chemoattractant protein-1 (MCP-1) and IL-8 are suggested to be prime candidates. In this study, we analyzed the presence of both chemokines in 24-h urinary (n = 15) and serum(n = 14) samples of HUS children by specific ELISAs. Furthermore, kidney biopsies of three different HUS children were examined for MO and PMN cell infiltration by histochemical techniques and electron microscopy. Whereas the chemokines MCP-1 and IL-8 were present in only very limited amounts in urine of 17 normal control subjects, serial samples of HUS patients demonstrated significantly elevated levels of both chemokines. HUS children with anuria showed higher initial and maximum chemokine levels than their counterparts without anuria. A strong positive correlation was observed between urinary MCP-1 and IL-8 levels. Whereas initial serum IL-8 levels were significantly increased in HUS children, serum MCP-1 levels were only slightly elevated compared with serum MCP-1 in control children. No correlation was found between urinary and serum chemokine concentrations. Histologic and EM studies of HUS biopsy specimens clearly showed the presence of MOs and to a lesser extent of PMNs in the glomeruli. The present data suggest an important local role for MOs and PMNs in the process of glomerular endothelial-cell damage. The chemokines MCP-1 and IL-8 may possibly be implicated in the pathogenesis of HUS through the recruitment and activation of MOs and PMNs, respectively.
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外周血单核细胞产生 IL-8 和单核细胞趋化肽-1。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Liebler,JM;Kunkel,SL;Burdick,MD;Standiford,TJ;Rolfe,MW;Strieter,RM
通讯作者：
Strieter,RM
影响因子：
13.2
作者：
Rovin, BH;Doe, N;Tan, LC
通讯作者：
Tan, LC
影响因子：
--
作者：
LAMAS, S;MICHEL, T;MARSDEN, PA
通讯作者：
MARSDEN, PA
巨噬细胞在肾小球损伤中的作用。
影响因子：
3.3
作者：
Schreiner,GF
通讯作者：
Schreiner,GF