Assignment1 of the feline Fas ligand gene (TNFSF6) to chromosome F1q12→q13 by fluorescence in situ hybridization
Assignment1 of the feline Fas ligand gene (TNFSF6) to chromosome F1q12→q13 by fluorescence in situ hybridization
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通过荧光原位杂交将猫 Fas 配体基因 (TNFSF6) 分配到染色体 F1q12→q13
DOI：
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发表时间：
2001
影响因子：
1.7
通讯作者：
H. Tsujimoto
中科院分区：
文献类型：
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作者：
Y. Fujino;T. Mizuno;K. Masuda;K. Ohno;H. Satoh;H. Tsujimoto
Fas配体（Fas ligand，FasL）是肿瘤坏死因子家族细胞因子的一员，通过与Fas受体结合诱导细胞凋亡。FasL主要在自然杀伤（NK）细胞和活化的T淋巴细胞上表达，并且也在免疫豁免位点如眼睛和睾丸中组成性表达（Nagata，1997）。在人类中，FasL介导的细胞死亡在调节淋巴细胞稳态以及对病毒和肿瘤细胞的免疫应答中具有关键作用;其失调与各种自身免疫性疾病（Nagata，1997）和由病毒感染引起的免疫缺陷的发病机制相关（Geleziunas et al.，2001年）。类似地，据报道，组成型表达FasL的各种肿瘤被提出来反击细胞毒性淋巴细胞并因此逃避特异性免疫监视（Strand et al.，1996年）。此外，在患有大颗粒淋巴细胞白血病和NK细胞淋巴瘤的患者的血清中已经观察到可引起全身组织损伤的可溶性形成的FasL的水平升高（Tanaka et al.，1996年）。对FasL基因在免疫介导的疾病、免疫缺陷和恶性肿瘤中的表达进行分析，将为阐明其病因和分子发病机制提供线索，并可能具有预后和治疗意义。在猫中，细胞凋亡已显示与免疫介导的疾病（Center，1999）、病毒性疾病（Ohno et al.，1993; Rojko等人，1996）和恶性肿瘤（Anilkumar等人，1994年）。为了提供猫FasL基因的基本细胞遗传学信息，我们用荧光原位杂交技术将猫FasL基因（TNFSF 6）定位于染色体F1 q12 →q13。有趣的是，在猫白血病病毒阳性和阴性淋巴瘤中观察到猫染色体F1的单体性和缺失（Gulino，1992），并且在患有纤维肉瘤的猫中检测到大标记染色体F1（Mayr等人，1996年）。因此，目前的数据将有助于研究猫的骨化相关疾病的分子发病机制，也了解比较细胞遗传学。由于人FASL定位在1 q23上（Takahashi et al.，1994），猫FasL是第一个证明猫染色体F1和人染色体1长臂之间同线性保守性的基因座（奥布莱恩等人，1997年）。
Fas ligand (FasL) is a member of the tumor necrosis factor family cytokines and induces apoptosis by binding to its receptor, Fas. FasL is predominantly expressed on natural killer (NK) cells and activated T lymphocytes, and also constitutively expressed in immune-privileged sites such as the eye and testis (Nagata, 1997). In human, FasL-mediated cell death has a crucial role in the regulation of lymphocyte homeostasis, and the immune response to viruses and tumor cells; its deregulation is associated with the pathogenesis of various autoimmune diseases (Nagata, 1997) and immunodeficiency caused by virus infection (Geleziunas et al., 2001). Similarly, it is reported that various tumors expressing FasL constitutively are proposed to counterattack the cytotoxic lymphocytes and consequently evade specific immune surveillance (Strand et al., 1996). Further, an elevated level of the soluble-formed FasL which may cause systemic tissue damage has been observed in sera of the patients with large granular lymphocytic leukemia and NK cell lymphoma (Tanaka et al., 1996). Analysis of the FasL gene in immune-mediated diseases, immunodeficiency and malignant tumors will provide a clue to the etiology and molecular pathogenesis, and may have prognostic and therapeutic implications. In cats, apoptosis has been shown to be associated with the pathogenesis of immune-mediated diseases (Center, 1999), viral diseases (Ohno et al., 1993; Rojko et al., 1996) and malignant tumors (Anilkumar et al., 1994). In order to provide basic cytogenetic information on the FasL gene in the cat, we regionally assigned the feline FasL gene (TNFSF6) to chromosome F1q12→q13 by fluorescence in situ hybridization. Interestingly, monosomy and deletion of feline chromosome F1 were observed in feline leukemia virus-positive and negative lymphomas (Gulino, 1992), and a large marker chromosome F1 was detected in a cat with fibrosarcoma (Mayr et al., 1996). Therefore the present data will help to investigate molecular pathogenesis of apoptosis-related diseases in cats and also to understand comparative cytogenetics. Since the human FASL was mapped on 1q23 (Takahashi et al., 1994), the feline FasL is the first gene locus proving conservation of synteny between the feline chromosome F1 and the long arm of human chromosome 1 (O’Brien et al., 1997).
猫白血病的染色体异常和肿瘤发生。
影响因子：
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作者：
Gulino,SE
通讯作者：
Gulino,SE
细胞病变猫白血病病毒导致血淋巴细胞凋亡。
DOI：
10.1007/978-3-642-79850-4_2
发表时间：
1996
期刊：
Progress in molecular and subcellular biology.
影响因子：
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作者：
Rojko,JL;Hartke,JR;Cheney,CM;Phipps,AJ;Neil,JC
通讯作者：
Neil,JC