A live-attenuated chlamydial vaccine protects against trachoma in nonhuman primates.
A live-attenuated chlamydial vaccine protects against trachoma in nonhuman primates.
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DOI：
10.1084/jem.20111266
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发表时间：
2011-10-24
期刊：
影响因子：
--
通讯作者：
Caldwell HD
中科院分区：
文献类型：
--
作者：
Kari L;Whitmire WM;Olivares-Zavaleta N;Goheen MM;Taylor LD;Carlson JH;Sturdevant GL;Lu C;Bakios LE;Randall LB;Parnell MJ;Zhong G;Caldwell HD
在食蟹猕猴中，缺乏保守的7.5 kb质粒的活沙眼感染没有引起眼部病理，但促进了对后续沙眼毒株感染的固体或部分保护。致盲性沙眼是由沙眼衣原体引起的一种古老的被忽视的热带病，需要疫苗。我们描述了一种安全有效地预防非人类灵长类动物沙眼的减毒活疫苗，这是一种对人类具有极好预测价值的模型。食蟹猴感染了一种缺乏7.5 kb保守质粒的沙眼病毒株后，出现了短暂的感染，感染后会自发消失，没有眼部病理。多次感染减毒质粒缺陷菌株不会引起眼部炎症，但会引起抗衣原体免疫反应。接种了减毒株的猕猴在受到携带毒质粒的生物体攻击后，要么得到了完全保护，要么得到了部分保护。受到部分保护的猕猴体内的传染性微生物明显少于对照组。保护性免疫的免疫相关因素尚未确定，但我们确实检测到了MHC II类等位基因与固体或部分保护之间的相关性。沙眼控制的流行病学模型表明，具有这种效力的疫苗将显著降低感染流行率和再感染率，这是导致致盲疾病的已知危险因素。
In cynomolgus macaques, ocular infection with a live trachoma strain lacking the conserved 7.5-kb plasmid induced no ocular pathology but facilitated solid or partial protection from subsequent infection with a virulent strain of trachoma. Blinding trachoma is an ancient neglected tropical disease caused by Chlamydia trachomatis for which a vaccine is needed. We describe a live-attenuated vaccine that is safe and efficacious in preventing trachoma in nonhuman primates, a model with excellent predictive value for humans. Cynomolgus macaques infected ocularly with a trachoma strain deficient for the 7.5-kb conserved plasmid presented with short-lived infections that resolved spontaneously without ocular pathology. Multiple infections with the attenuated plasmid-deficient strain produced no inflammatory ocular pathology but induced an anti-chlamydial immune response. Macaques vaccinated with the attenuated strain were either solidly or partially protected after challenge with virulent plasmid-bearing organisms. Partially protected macaques shed markedly less infectious organisms than controls. Immune correlates of protective immunity were not identified, but we did detect a correlation between MHC class II alleles and solid versus partial protection. Epidemiological models of trachoma control indicate that a vaccine with this degree of efficacy would significantly reduce the prevalence of infection and rates of reinfection, known risk factors which drive blinding disease.
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影响因子：
9.8
作者：
Rodgers AK;Wang J;Zhang Y;Holden A;Berryhill B;Budrys NM;Schenken RS;Zhong G
通讯作者：
Zhong G
影响因子：
158.5
作者：
SCHACHTER, J
通讯作者：
SCHACHTER, J
DOI：
10.4049/jimmunol.0804375
发表时间：
2009-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kari L;Whitmire WM;Crane DD;Reveneau N;Carlson JH;Goheen MM;Peterson EM;Pal S;de la Maza LM;Caldwell HD
通讯作者：
Caldwell HD
影响因子：
3.1
作者：
SU, H;WATKINS, NG;CALDWELL, HD
通讯作者：
CALDWELL, HD
影响因子：
4.2
作者：
Bailey, R;Duong, T;Mabey, D
通讯作者：
Mabey, D