Serial Analysis of Gene Expression (SAGE)

Serial Analysis of Gene Expression (SAGE)
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基因表达系列分析 (SAGE)

DOI:
10.1002/0471142727.mb25b06s61
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发表时间:
2003
影响因子:
--
通讯作者:
K. Polyak
K. Polyak
中科院分区:
--
文献类型:
--
作者:
S. Blackshaw;Jae Bum Kim;B. S. Croix;K. Polyak

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基因表达系列分析(SAGE)涉及从分析样品中所有cDNA序列中的定义点产生DNA的短片段或标签。由于其在序列中的确定存在,该短标签通常足以唯一地鉴定样品中的每个转录物。因此,可以生成任何样品中基因表达的综合概况。基因表达系列分析(SAGE)是指从一个特定的点产生DNA的短片段或标签。基因表达系列分析(SAGE)是指从一个特定的点产生DNA的短片段或标签。
Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed. This short tag, because of its defined presence in the sequence, is typically sufficient to uniquely identify every transcript in the sample. A comprehensive profile of gene expression in any sample can thus be generated. Absolute, rather than relative, measurements of RNA abundance levels are made, which will be useful in the reliable comparison of data between laboratories.Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point
DOI: 10.1126/science.276.5316.1268
发表时间: 1997-05-23
期刊: SCIENCE
影响因子: 56.9
作者:
Zhang, L;Zhou, W;Kinzler, KW
通讯作者: Kinzler, KW