Revealing a signaling role of phytosphingosine-1-phosphate in yeast.
Revealing a signaling role of phytosphingosine-1-phosphate in yeast.
复制标题
DOI：
10.1038/msb.2010.3
复制
发表时间：
2010
影响因子：
9.9
通讯作者：
Lu, Xinghua
中科院分区：
文献类型：
--
作者：
Cowart, L. Ashley;Shotwell, Matthew;Worley, Mitchell L.;Richards, Adam J.;Montefusco, David J.;Hannun, Yusuf A.;Lu, Xinghua
鞘磷脂包括鞘氨醇-1-磷酸和神经酰胺，通过信号机制参与许多细胞程序。这类脂类在应激反应中具有重要的功能；然而，确定哪种鞘脂介导特定事件仍然受到鞘脂之间众多代谢相互联系的阻碍，以至于通过操纵代谢酶来调节感兴趣的特定脂类会引起“连锁反应”，从而改变许多其他脂类的水平。在这里，我们开发了一种对基因组、转录体和脂体学数据进行综合分析的方法来解决这个以前难以解决的问题。这种方法揭示了植酸鞘氨醇-1-磷酸在通过HAP复合体转录因子调节线粒体呼吸所需基因中的特定信号作用。植鞘糖苷-1-磷酸是一种在酵母中先前没有定义的特定功能的脂类。这种方法可以应用于从产生多组高通量数据的类似实验设计中提取有意义的生物信息。
Sphingolipids including sphingosine-1-phosphate and ceramide participate in numerous cell programs through signaling mechanisms. This class of lipids has important functions in stress responses; however, determining which sphingolipid mediates specific events has remained encumbered by the numerous metabolic interconnections of sphingolipids, such that modulating a specific lipid of interest through manipulating metabolic enzymes causes ‘ripple effects’, which change levels of many other lipids. Here, we develop a method of integrative analysis for genomic, transcriptomic, and lipidomic data to address this previously intractable problem. This method revealed a specific signaling role for phytosphingosine-1-phosphate, a lipid with no previously defined specific function in yeast, in regulating genes required for mitochondrial respiration through the HAP complex transcription factor. This approach could be applied to extract meaningful biological information from a similar experimental design that produces multiple sets of high-throughput data.
登录
查看更多内容
影响因子：
12.3
作者：
Lee SI;Batzoglou S
通讯作者：
Batzoglou S
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
--
作者：
Buschlen, S;Amillet, JM;Bolotin-Fukuhara, M
通讯作者：
Bolotin-Fukuhara, M
DOI：
10.1073/pnas.2136632100
发表时间：
2003-12-23
影响因子：
11.1
作者：
Liao, JC;Boscolo, R;Roychowdhury, VP
通讯作者：
Roychowdhury, VP
影响因子：
4.8
作者：
Jenkins, GM;Hannun, YA
通讯作者：
Hannun, YA