Degradation in vivo of the C3 protein of guinea-pig complement by a pathogenic strain of Bacteroides gingivalis.
Degradation in vivo of the C3 protein of guinea-pig complement by a pathogenic strain of Bacteroides gingivalis.
复制标题
牙龈拟杆菌致病菌株对豚鼠补体 C3 蛋白的体内降解。
DOI：
10.1111/j.1600-0722.1984.tb00854.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
A. Väätäinen
中科院分区：
文献类型：
--
作者：
G. Sundqvist;J. Carlsson;B. Herrmann;J. F. Höfling;A. Väätäinen
在豚鼠皮下植入聚四氟乙烯笼中检测了5株黑色拟杆菌的致病性。记录融合器周围的组织反应，并分析融合器液体的含量。两株B.中间肌引起笼周围局部脓肿，而B. gingivalis和不同于B的溶砷菌株（BN 11 a-f）。牙龈炎没有引起任何脓肿形成的迹象。1株（W83）为B. B.牙龈炎引起组织的广泛化脓性破坏。当菌株W83的接种物含有超过10（9）个细胞时，处死动物。菌株W83是笼中数量增加的唯一菌株。接种W83菌株的网箱培养液与接种其他菌株的网箱培养液也有显著差异。该液体具有较高的蛋白水解活性。体液中未检测到补体C3蛋白，仅检测到微量免疫球蛋白。菌株W83和菌株381对豚鼠全血清都有较高的蛋白水解活性，当这两株细菌与豚鼠血清孵育24 h时，几乎所有的血清蛋白，包括C3蛋白，都被降解。因此，这两种菌株可能具有类似的能力，在干扰宿主防御接种到组织笼。菌株之间致病性的实际差异可能由最近的发现来解释，即致病菌株W83而不是菌株381在激活多形核白细胞时需要补体。研究了B.因此，牙龈炎可能是干扰宿主防御的关键事件。菌株381对C3蛋白的降解对逃避宿主防御没有帮助，因为该菌株在缺乏补体的情况下激活多形核白细胞。
The pathogenicity of five black-pigmented strains of Bacteroides was tested in subcutaneously implanted Teflon cages in guinea pigs. The tissue reaction around the cages was registered and the contents of the fluid of the cages were analyzed. Two strains of B. intermedius produced a localized abscess around the cages, while one strain (381) of B. gingivalis and an asaccharolytic strain (BN11a-f) different from B. gingivalis did not induce any signs of abscess formation. One strain (W83) of B. gingivalis caused extensive purulent breakdown of the tissues. When the inoculum of strain W83 contained more than 10(9) cells, the animals were killed. Strain W83 was the only strain that increased in number in the cage. The fluid of cages inoculated with strain W83 was also remarkably different from the fluid of cages inoculated with the other strains. The fluid had a high proteolytic activity. No C3 protein of complement and only traces of immunoglobulins could be detected in the fluid. Both strain W83 and strain 381 had a high proteolytic activity against whole guinea-pig serum and when bacteria of these two strains were incubated with guinea-pig serum for 24 h, almost all serum proteins, including the C3 protein, were degraded. These two strains might thus have similar capacity in perturbing the host defence when inoculated into the tissue cages. The actual difference in pathogenicity between the strains might be explained by a recent finding that the pathogenic strain W83, but not strain 381, requires complement in activating polymorphonuclear leukocytes. The degradation of the C3 protein by the pathogenic strain W83 of B. gingivalis thus may be the crucial event in its perturbation of the host defence. A degradation of the C3 protein by strain 381 would be of no help in eluding the host defence, since this strain activates polymorphonuclear leukocytes in the absence of complement.
影响因子：
3.5
作者：
Passo,SA;Tsai,CC;McArthur,WP;Leifer,C;Taichman,NS
通讯作者：
Taichman,NS