Sodium-glucose cotransporter 1 as a sugar taste sensor in mouse tongue.
Sodium-glucose cotransporter 1 as a sugar taste sensor in mouse tongue.
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DOI：
10.1111/apha.13529
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Ninomiya Y
中科院分区：
文献类型：
--
作者：
Yasumatsu K;Ohkuri T;Yoshida R;Iwata S;Margolskee RF;Ninomiya Y
我们研究了编码糖信息的潜在神经元类型以及钠-葡萄糖协同转运蛋白（SGLT）和T1 R如何参与。在T1 R3-KO和野生型（WT）小鼠中进行鼓索（CT）和舌咽（GL）神经的全神经记录和CT中的单纤维记录。使用根皮苷（Phl）（SGLT的竞争性抑制剂）在T1 R3-KO小鼠中进行行为反应测量。结果表明，在CT和GL神经中加入10 mmol/LNaCl后，对蔗糖和葡萄糖（Glc）的反应明显增强，而对KCl、谷氨酸单钾、柠檬酸、硫酸奎宁、SC 45647（SC）和多糖的反应无明显增强。这些增强被废除的Phl语言应用。在单纤维记录中，WT小鼠CT中对蔗糖反应最大的纤维可根据其对SC和Glc（含或不含10 mmol/L NaCl）的反应分为Phl不敏感型、Phl敏感型和混合型（Glc和SC反应）纤维。在T1 R3-KO小鼠中，Phl不敏感型纤维消失。行为学实验结果表明，Phl对Glc的舔食次数和摄食量（含或不含10 mmol/L NaCl）均有显著抑制作用。我们发现了SGLTs在小鼠舌味觉细胞糖感知中的贡献的证据。此外，我们还发现了T1 R依赖型（Phl不敏感型）、Glc型和混合型（SGLT和T1 R）纤维。SGLT 1可能参与后两种类型，并可能在葡萄糖特异性消化和可口食物摄入的头期中发挥重要作用。
We investigated potential neuron types that code sugar information and how sodium-glucose cotransporters (SGLTs) and T1Rs are involved. Whole-nerve recordings in the chorda tympani (CT) and the glossopharyngeal (GL) nerves and single-fibre recordings in the CT were performed in T1R3-KO and wild-type (WT) mice. Behavioural response measurements were conducted in T1R3-KO mice using phlorizin (Phl), a competitive inhibitor of SGLTs. Results indicated that significant enhancement occurred in responses to sucrose and glucose (Glc) by adding 10 mmol/L NaCl but not in responses to KCl, monopotassium glutamate, citric acid, quinine sulphate, SC45647(SC) or polycose in both CT and GL nerves. These enhancements were abolished by lingual application of Phl. In single-fibre recording, fibres showing maximal response to sucrose could be classified according to responses to SC and Glc with or without 10 mmol/L NaCl in the CT of WT mice, namely, Phl-insensitive type, Phl-sensitive Glc-type and Mixed (Glc and SC responding)-type fibres. In T1R3-KO mice, Phl-insensitive-type fibres disappeared. Results from behavioural experiments showed that the number of licks and amount of intake for Glc with or without 10 mmol/L NaCl were significantly suppressed by Phl. We found evidence for the contribution of SGLTs in sugar sensing in taste cells of mouse tongue. Moreover, we found T1R-dependent (Phl-insensitive) type, Glc-type and Mixed (SGLTs and T1Rs)-type fibres. SGLT1 may be involved in the latter two types and may play important roles in the glucose-specific cephalic phase of digestion and palatable food intake.
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DOI：
10.1152/ajpregu.00056.2015
发表时间：
2015-09-15
影响因子：
2.8
作者：
Glendinning, John I.;Stano, Sarah;Sclafani, Anthony
通讯作者：
Sclafani, Anthony
影响因子：
2.9
作者：
NINOMIYA, Y;HIGASHI, T;FUNAKOSHI, M
通讯作者：
FUNAKOSHI, M
影响因子：
8.2
作者：
Parker, H. E.;Adriaenssens, A.;Rogers, G.;Richards, P.;Koepsell, H.;Reimann, F.;Gribble, F. M.
通讯作者：
Gribble, F. M.
影响因子：
3.9
作者：
Raja，Mobeen;Kinne，Rolf K. H.
通讯作者：
Kinne，Rolf K. H.
影响因子：
6.9
作者：
Glendinning, John I.
通讯作者：
Glendinning, John I.