Coordinated circadian timing through the integration of local inputs in Arabidopsis thaliana
Coordinated circadian timing through the integration of local inputs in Arabidopsis thaliana
复制标题
通过整合拟南芥的本地投入来协调昼夜节律
DOI：
10.1101/617803
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Greenwood M
中科院分区：
文献类型：
--
作者：
Greenwood M
单个植物细胞有一个遗传回路，即生物钟，它为昼夜循环的关键过程计时。这些时钟通过在整个工厂中变化的多个环境信号与昼夜周期对齐。植物如何整合器官内部和器官之间的时钟节奏，以确保协调的时间？为了解决这个问题，我们在亚组织水平上研究了拟南芥幼苗在多种环境条件和遗传背景下的生物钟。我们的研究结果表明，时钟在每个器官中以不同的速度（周期）运行，这导致时钟在恒定的环境条件和光暗（LD）周期中在整个植物的不同时间达到峰值。更仔细的研究表明，时钟基因表达的空间波在器官内部和器官之间传播。使用建模和实验相结合，我们揭示了这些空间波是器官之间的周期差异和局部耦合的结果，而不是长距离信号。进一步的实验表明，内源性周期差异，从而空间波，可以产生的器官特异性输入到时钟。我们通过使用光和代谢信号以及遗传扰动来调节周期来证明这一点。我们的研究结果表明，植物时钟可以设置本地的器官特异性输入，但协调全球通过时钟基因表达的空间波。
Individual plant cells have a genetic circuit, the circadian clock, that times key processes to the day-night cycle. These clocks are aligned to the day-night cycle by multiple environmental signals that vary across the plant. How does the plant integrate clock rhythms, both within and between organs, to ensure coordinated timing? To address this question, we examined the clock at the sub-tissue level acrossArabidopsis thalianaseedlings under multiple environmental conditions and genetic backgrounds. Our results show that the clock runs at different speeds (periods) in each organ, which causes the clock to peak at different times across the plant in both constant environmental conditions and light-dark (LD) cycles. Closer examination reveals that spatial waves of clock gene expression propagate both within and between organs. Using a combination of modeling and experiment, we reveal that these spatial waves are the result of the period differences between organs and local coupling, rather than long-distance signaling. With further experiments we show that the endogenous period differences, and thus the spatial waves, can be generated by the organ specificity of inputs into the clock. We demonstrate this by modulating periods using light and metabolic signals, as well as with genetic perturbations. Our results reveal that plant clocks can be set locally by organ-specific inputs but coordinated globally via spatial waves of clock gene expression.
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影响因子：
4.4
作者：
Pett JP;Kondoff M;Bordyugov G;Kramer A;Herzel H
通讯作者：
Herzel H
怀拉基的昼夜和季节性地面温度变化
影响因子：
2.2
作者：
G. Dawson;R. G. Fisher
通讯作者：
R. G. Fisher
DOI：
10.1007/978-1-4419-1262-6
发表时间：
2010
期刊：
--
影响因子：
--
作者：
通讯作者：
--
最小二乘分析
DOI：
--
发表时间：
2005
期刊：
影响因子：
--
作者：
Thomas B. Barker
通讯作者：
Thomas B. Barker
影响因子：
7.4
作者：
Andrea Palágyi;Kata Terecskei;É. Ádám;E. Kevei;S. Kircher;Zsuzsanna Mérai;Eberhard Schäfer;F. Nagy;László Kozma-Bognár
通讯作者：
Andrea Palágyi;Kata Terecskei;É. Ádám;E. Kevei;S. Kircher;Zsuzsanna Mérai;Eberhard Schäfer;F. Nagy;László Kozma-Bognár