PML protein analysis using imaging flow cytometry
PML protein analysis using imaging flow cytometry
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使用成像流式细胞术分析 PML 蛋白
DOI：
10.1136/jcp.2010.085662
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发表时间：
2010
影响因子：
3.4
通讯作者：
W. Erber
中科院分区：
文献类型：
--
作者：
L. Grimwade;E. Gudgin;D. Bloxham;M. Scott;W. Erber
急性早幼粒细胞白血病（APML）可通过免疫荧光显微镜检测异常早幼粒细胞中PML小体的异常弥漫染色模式来迅速诊断。然而，这种技术是主观的，灵敏度低。使用来自18名急性髓性白血病（AML）患者（包括4名APML患者）的样本，作者研究了成像流式细胞术是否可以成为当前技术的可行替代方案并提高灵敏度水平。用PML抗体对骨髓/外周血细胞进行染色，并在ImageStream（Amnis Corporation，西雅图，华盛顿，美国）上获取数据。使用调制特征进行数据分析，作者证明该技术可以成功识别APML病例。成像流式细胞术通过分析更多数量的细胞并具有包括疾病特异性抗原的潜力，增加了当前免疫荧光技术的灵敏度。成像流式细胞术是一项令人兴奋的技术，在血液系统恶性肿瘤的诊断中有许多可能的应用，包括整合模式的潜力。
Acute promyelocytic leukaemia (APML) can be promptly diagnosed by detecting abnormal diffuse staining patterns of PML bodies in abnormal promyelocytes using immunofluorescence microscopy. However, this technique is subjective, with low sensitivity. Using samples from 18 patients with acute myeloid leukaemia (AML) (including four with APML), the authors investigated whether imaging flow cytometry could be a viable alternative to this current technique and improve sensitivity levels. Bone marrow/peripheral blood cells were stained with an antibody to PML, and data were acquired on an ImageStream (Amnis Corporation, Seattle, Washington, USA). Using the modulation feature for data analysis, the authors demonstrated that this technique could successfully identify cases of APML. Imaging flow cytometry, by analysing greater numbers of cells and with the potential to include disease-specific antigens, increases the sensitivity of the current immunofluorescence technique. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of haematological malignancies, including the potential to integrate modalities.
影响因子：
1.7
作者：
Basiji, David A.;Ortyn, William E.;Morrissey, Philip
通讯作者：
Morrissey, Philip