Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus
Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus
复制标题
针对恒河猴轮状病毒两种表面蛋白的单克隆抗体的制备和初步表征
作者：
H. B. Greenberg;J. Valdesuso;K. V. van Wyke;K. Midthun;M. Walsh;V. McAULIFFE;R G Wyatt;A. Kalica;J. Flores;Y. Hoshino
分离到一系列与恒河猴轮状病毒两种主要表面蛋白之一发生反应的单抗。36个单抗免疫沉淀了82千道尔顿的外衣壳蛋白，这是第四个基因病毒血凝素的产物。这些单抗具有血凝抑制活性，能中和中、高滴度的恒河猴轮状病毒。三种单抗免疫沉淀了38千道尔顿的外衣壳糖蛋白，这是第八或第九个基因产物。这三种单抗中和了恒河猴轮状病毒的高滴度，也抑制了病毒的血凝。
A series of monoclonal antibodies was isolated which reacted with one of two major surface proteins of rhesus rotavirus. Thirty-six monoclonal antibodies immunoprecipitated the 82-kilodalton outer capsid protein, the product of the fourth gene, the viral hemagglutinin. These monoclonal antibodies exhibited hemagglutination inhibition activity and neutralized rhesus rotavirus to moderate or high titer. Three monoclonal antibodies immunoprecipitated the 38-kilodalton outer capsid glycoprotein, the eighth or ninth gene product. These three monoclonal antibodies neutralized rhesus rotavirus to high titer and also inhibited viral hemagglutination.
一系列杂交瘤 IgG 与呼肠孤病毒颗粒的相互作用。
影响因子：
3.7
作者：
Hayes,EC;Lee,PW;Miller,SE;Joklik,WK
通讯作者：
Joklik,WK
影响因子：
3.7
作者：
LEE, PWK;HAYES, EC;JOKLIK, WK
通讯作者：
JOKLIK, WK