Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system.
Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system.
复制标题
DOI：
10.1186/1475-2859-4-14
复制
发表时间：
2005-05-16
影响因子：
6.4
通讯作者：
Gosset G
中科院分区：
文献类型：
--
作者：
Gosset G
代谢工程在大肠杆菌中的应用已经导致产生具有产生商业利益的代谢物的能力的菌株。使用这些工程菌株的生物技术过程经常使用含有葡萄糖作为碳源和能源的培养基。在大肠在大肠杆菌中，磷酸烯醇丙酮酸：糖磷酸转移酶系统（PTS）在葡萄糖以类似于生产发酵中使用的浓度存在时转运葡萄糖。该蛋白质系统参与磷酸烯醇式丙酮酸依赖的糖转运，因此，其活性对磷酸烯醇式丙酮酸和丙酮酸节点中的碳通量分布具有重要影响。此外，PTS在碳分解代谢物阻遏中具有非常重要的作用。PTS的性质施加了可能阻碍菌株生产力的代谢和调节约束。因此，PTS一直是以菌株改良为目的的改性目标。在这篇综述中，PTS特性最相关的应变性能和不同的策略，PTS的改造，以提高应变进行了讨论。通过替代的磷酸烯醇化酸非依赖性摄取和磷酸化活性对PTS进行功能性替代，显著提高了葡萄糖的产物产率和几类代谢物的生产率。此外，PTS组分的失活已被成功地用作消除碳代谢产物阻遏的策略，导致E.更有效地利用糖混合物的大肠杆菌菌株，如从木质纤维素水解产物获得的那些。
The application of metabolic engineering in Escherichia coli has resulted in the generation of strains with the capacity to produce metabolites of commercial interest. Biotechnological processes with these engineered strains frequently employ culture media containing glucose as the carbon and energy source. In E. coli, the phosphoenolpyruvate:sugar phosphotransferase system (PTS) transports glucose when this sugar is present at concentrations like those used in production fermentations. This protein system is involved in phosphoenolpyruvate-dependent sugar transport, therefore, its activity has an important impact on carbon flux distribution in the phosphoenolpyruvate and pyruvate nodes. Furthermore, PTS has a very important role in carbon catabolite repression. The properties of PTS impose metabolic and regulatory constraints that can hinder strain productivity. For this reason, PTS has been a target for modification with the purpose of strain improvement. In this review, PTS characteristics most relevant to strain performance and the different strategies of PTS modification for strain improvement are discussed. Functional replacement of PTS by alternative phosphoenolpyruvate-independent uptake and phosphorylation activities has resulted in significant improvements in product yield from glucose and productivity for several classes of metabolites. In addition, inactivation of PTS components has been applied successfully as a strategy to abolish carbon catabolite repression, resulting in E. coli strains that use more efficiently sugar mixtures, such as those obtained from lignocellulosic hydrolysates.
登录
查看更多内容
影响因子：
4.1
作者：
FORBERG, C;ELIAESON, T;HAGGSTROM, L
通讯作者：
HAGGSTROM, L
影响因子：
5
作者：
Hernández-Montalvo, V;Valle, F;Gosset, G
通讯作者：
Gosset, G
影响因子：
3.8
作者：
HAN, K;LIM, HC;HONG, J
通讯作者：
HONG, J
影响因子：
2.9
作者：
Bothast, RJ;Nichols, NN;Dien, BS
通讯作者：
Dien, BS
影响因子：
3.2
作者：
CURTIS, SJ;EPSTEIN, W
通讯作者：
EPSTEIN, W