Circadian regulation of response to oxidative stress in Drosophila melanogaster.
Circadian regulation of response to oxidative stress in Drosophila melanogaster.
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DOI：
10.1016/j.bbrc.2008.07.011
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发表时间：
2008-09-19
影响因子：
3.1
通讯作者：
Giebultowicz, Jadwiga M.
中科院分区：
文献类型：
--
作者：
Krishnan, Natraj;Davis, Andrew J.;Giebultowicz, Jadwiga M.
昼夜节律是由称为生物钟的分子遗传机制产生的基本生物现象。越来越多的证据表明，生理和细胞过程的昼夜节律同步有助于生物体的健康，抑制癌症和过早衰老等病理。因此，有必要了解生物钟如何协调有机体内部过程和环境之间的相互作用。在这里，我们探讨果蝇的时钟和氧化应激易感性之间的联系。我们将苍蝇暴露于过氧化氢（H2 O2）诱导的急性氧化应激，并确定死亡率取决于暴露发生在白天/黑夜周期的时间。在核心时钟基因周期（每）废除时钟功能的无效突变的果蝇中，每日易感性节律被废除。此外，与野生型果蝇相比，缺乏per增加了对H2 O2的敏感性，这与线粒体H2 O2的产生增加和氧化损伤导致的过氧化氢酶活性降低一致。总之，我们的数据表明，生物钟基因周期对于维持强大的抗氧化防御至关重要。
Circadian rhythms are fundamental biological phenomena generated by molecular genetic mechanisms known as circadian clocks. There is increasing evidence that circadian synchronization of physiological and cellular processes contribute to the wellness of organisms, curbing pathologies such as cancer and premature aging. Therefore, there is a need to understand how circadian clocks orchestrate interactions between the organism's internal processes and the environment. Here, we explore the nexus between the clock and oxidative stress susceptibility in Drosophila melanogaster. We exposed flies to acute oxidative stress induced by hydrogen peroxide (H2O2), and determined that mortality rates were dependent on time at which exposure occurred during the day /night cycle. The daily susceptibility rhythm was abolished in flies with a null mutation in the core clock gene period (per) abrogating clock function. Furthermore, lack of per increased susceptibility to H2O2 compared to wild-type flies, coinciding with enhanced generation of mitochondrial H2O2 and decreased catalase activity due to oxidative damage. Taken together, our data suggest that the circadian clock gene period is essential for maintaining a robust anti-oxidative defense.
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影响因子：
7.4
作者：
McClung, CR
通讯作者：
McClung, CR
DOI：
10.1073/pnas.0409897102
发表时间：
2005-03-01
影响因子：
11.1
作者：
Gorbacheva, VY;Kondratov, RV;Antoch, MP
通讯作者：
Antoch, MP
影响因子：
10.5
作者：
Kondratov, Roman V.;Kondratova, Anna A.;Antoch, Marina P.
通讯作者：
Antoch, Marina P.
影响因子：
1.1
作者：
Coto-Montes, A;Hardeland, R
通讯作者：
Hardeland, R
DOI：
10.1098/rstb.2001.0960
发表时间：
2001-11-29
影响因子：
6.3
作者：
Giebultowicz, JM
通讯作者：
Giebultowicz, JM