Mapping the tail fiber as the receptor binding protein responsible for differential host specificity of Pseudomonas aeruginosa bacteriophages PaP1 and JG004.
Mapping the tail fiber as the receptor binding protein responsible for differential host specificity of Pseudomonas aeruginosa bacteriophages PaP1 and JG004.
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将尾部纤维映射为负责铜绿假单胞菌噬菌体 PaP1 和 JG004 差异宿主特异性的受体结合蛋白
DOI：
10.1371/journal.pone.0068562
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu F
中科院分区：
文献类型：
--
作者：
Le S;He X;Tan Y;Huang G;Zhang L;Lux R;Shi W;Hu F
噬菌体感染的第一步是识别并结合宿主受体，这是由噬菌体受体结合蛋白（RBP）介导的。不同的RBP可导致不同的宿主特异性。在许多噬菌体中，如大肠杆菌和乳球菌，RBPs已被鉴定为尾纤维或突出的基板蛋白。然而，铜绿假单胞菌尾纤依赖的宿主特异性尚未得到很好的研究。本研究旨在鉴定和研究铜绿假单胞菌RBP与JG004的结合特异性。这两种DNA序列具有高度同源性，但表现出不同的宿主特异性。一个自发突变的噬菌体被分离出来，并表现出更广泛的宿主范围相比，亲本噬菌体JG004。其推测的尾纤和底板区域的测序表明ORF 84（推测的尾纤基因）中的单点突变，其导致带正电荷的赖氨酸（K）被不带电荷的天冬酰胺（N）取代。我们进一步证明了用来自噬菌体JG004的相应基因替换PaP1的尾纤基因（ORF 69）导致了显示改变的宿主特异性的重组噬菌体。我们的研究揭示了铜绿假单胞菌尾纤依赖的宿主特异性，并为其改变提供了有效的工具。这些贡献可能在噬菌体治疗中具有潜在价值。
The first step in bacteriophage infection is recognition and binding to the host receptor, which is mediated by the phage receptor binding protein (RBP). Different RBPs can lead to differential host specificity. In many bacteriophages, such as Escherichia coli and Lactococcal phages, RBPs have been identified as the tail fiber or protruding baseplate proteins. However, the tail fiber-dependent host specificity in Pseudomonas aeruginosa phages has not been well studied. This study aimed to identify and investigate the binding specificity of the RBP of P. aeruginosa phages PaP1 and JG004. These two phages share high DNA sequence homology but exhibit different host specificities. A spontaneous mutant phage was isolated and exhibited broader host range compared with the parental phage JG004. Sequencing of its putative tail fiber and baseplate region indicated a single point mutation in ORF84 (a putative tail fiber gene), which resulted in the replacement of a positively charged lysine (K) by an uncharged asparagine (N). We further demonstrated that the replacement of the tail fiber gene (ORF69) of PaP1 with the corresponding gene from phage JG004 resulted in a recombinant phage that displayed altered host specificity. Our study revealed the tail fiber-dependent host specificity in P. aeruginosa phages and provided an effective tool for its alteration. These contributions may have potential value in phage therapy.
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影响因子：
2.9
作者：
Heineman, Richard H.;Springman, Rachael;Bull, James J.
通讯作者：
Bull, James J.
DOI：
10.1073/pnas.1011218107
发表时间：
2010-11-23
影响因子：
11.1
作者：
Bartual, Sergio G.;Otero, Jose M.;van Raaij, Mark J.
通讯作者：
van Raaij, Mark J.
影响因子：
4.9
作者：
Qin, Xuan;Zerr, Danielle M.;Kapur, Raj P.
通讯作者：
Kapur, Raj P.
影响因子：
3.7
作者：
HELLER, KJ
通讯作者：
HELLER, KJ
影响因子：
4.9
作者：
McVay, Catherine S.;Velasquez, Marisela;Fralick, Joe A.
通讯作者：
Fralick, Joe A.