Osmorespiratory Compromise in Zebrafish (Danio rerio): Effects of Hypoxia and Acute Thermal Stress on Oxygen Consumption, Diffusive Water Flux, and Sodium Net Loss Rates.
Osmorespiratory Compromise in Zebrafish (Danio rerio): Effects of Hypoxia and Acute Thermal Stress on Oxygen Consumption, Diffusive Water Flux, and Sodium Net Loss Rates.
复制标题
斑马鱼（斑马鱼）的渗透呼吸损害：缺氧和急性热应激对耗氧量、扩散水通量和钠净损失率的影响。
作者：
J. Onukwufor;C. Wood
传统的鳃呼吸系统损害的论点是，低鳃水和离子渗透性将在O2需求升高时被交换为增加的O2渗透性。然而，有越来越多的证据表明，这些渗透性的独立调节耐缺氧的鱼类。使用先前在25°C下保持在常氧下的0.5 g斑马鱼，我们研究了对急性温度挑战（15°C或35°C），急性缺氧（10%或5%空气饱和度下15 min）以及长期暴露于10%缺氧，O2消耗（MO2），扩散水通量和净钠损失率的反应。暴露于35°C增加，15°C降低所有三种速率，扩散水通量显示出最低的温度敏感性，Na+损失最大。急性10%和5%缺氧增加了弥散性水通量和净Na+损失，并减少了MO2。所有这些反应都反映了传统的呼吸道损害。然而，在长时间的10%缺氧，MO2恢复，扩散水通量低于控制水平下降，和Na+损失率仍然升高，即使在posthypoxia恢复。总的来说，斑马鱼不符合标准模式，以前看到的缺氧耐受或不耐受的鱼，但显然能够调整有效的渗透性，他们的鳃O2，水和离子独立在急性温度和缺氧暴露。
The traditional thesis of the osmorespiratory compromise is that low branchial water and ion permeability would be traded off for increased O2 permeability at times of elevated O2 demand. However, there is growing evidence of independent regulation of these permeabilities in hypoxia-tolerant fish. Using 0.5-g zebrafish previously maintained under normoxia at 25°C, we investigated responses to acute temperature challenges (15°C or 35°C), acute hypoxia (15 min at 10% or 5% air saturation), as well as longer-term exposures to 10% hypoxia, on O2 consumption (MO2), diffusive water flux, and net sodium loss rates. Exposure to 35°C increased, and 15°C decreased all three rates, with diffusive water flux showing the lowest temperature sensitivity, and Na+ loss the greatest. Acute 10% and 5% hypoxia increased diffusive water flux and net Na+ loss, and it reduced MO2. All these responses reflected the traditional osmorespiratory compromise. However, during prolonged 10% hypoxia, MO2 recovered, diffusive water flux decreased below control levels, and Na+ loss rate remained elevated, even during posthypoxia recovery. Overall, zebrafish do not fit standard patterns previously seen in either hypoxia-tolerant or -intolerant fish but are clearly able to adjust the effective permeabilities of their gills to O2, water, and ions independently during acute temperature and hypoxia exposures.
在淡水或海水中，水通道蛋白 3 是否参与缺氧和常氧恢复期间鳉鱼的水渗透性变化？
DOI：
10.1002/jez.2393
发表时间：
2020
期刊：
Journal of experimental zoology. Part A, Ecological and integrative physiology
影响因子：
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作者：
Ruhr,IlanM;Wood,ChrisM;Schauer,KevinL;Wang,Yadong;Mager,EdwardM;Stanton,Bruce;Grosell,Martin
通讯作者：
Grosell,Martin