A genome-wide approach reveals novel imprinted genes expressed in the human placenta.
A genome-wide approach reveals novel imprinted genes expressed in the human placenta.
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DOI：
10.4161/epi.21495
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发表时间：
2012-09
期刊：
影响因子：
3.7
通讯作者：
Vaiman D
中科院分区：
文献类型：
--
作者：
Barbaux S;Gascoin-Lachambre G;Buffat C;Monnier P;Mondon F;Tonanny MB;Pinard A;Auer J;Bessières B;Barlier A;Jacques S;Simeoni U;Dandolo L;Letourneur F;Jammes H;Vaiman D
基因组印记是指基因的单等位基因表达，其表达依赖于每个等位基因的亲本来源。迄今为止，在人类和小鼠中已知大约150个印记基因，但是，尽管计算机搜索试图提取这些基因的内在特征以识别新的基因，但现有的列表可能远远不够全面。我们使用高通量策略，通过转移基因分型微阵列的经典用途来比较mRNA/cDNA与基因组DNA的基因型，以从人胎盘材料开始鉴定呈现单等位基因表达的新基因。在过滤数据后，我们获得了位于100多个候选基因中的1，082个推定的候选单等位基因SNP的列表。其中，我们发现了已知的印迹基因，如IPW，GRB 10，INPP 5 F和ZNF 597，这有助于验证该方法。我们还对可能的候选基因进行了研究，发现了7个新的印迹基因，包括ZFAT、ZFAT-AS 1、GLIS 3、NTM、MAGI 2、ZC 3 H12 C和LIN 28 B，其中4个编码锌指转录因子。然而，除了Magi 2外，它们不印在小鼠胎盘中。我们更详细地分析了ZFAT基因，该基因在胎盘中父系表达（作为ZFAT-AS 1，一种非编码反义RNA），但在其他组织中是双等位基因。ZFAT蛋白在内皮细胞以及合体滋养层中表达。此外，该基因的表达在复杂妊娠的胎盘中下调。通过这项工作，我们将人类已知的印记基因数量增加了约10%。
Genomic imprinting characterizes genes with a monoallelic expression, which is dependent on the parental origin of each allele. Approximately 150 imprinted genes are known to date, in humans and mice but, though computational searches have tried to extract intrinsic characteristics of these genes to identify new ones, the existing list is probably far from being comprehensive. We used a high-throughput strategy by diverting the classical use of genotyping microarrays to compare the genotypes of mRNA/cDNA vs. genomic DNA to identify new genes presenting monoallelic expression, starting from human placental material. After filtering of data, we obtained a list of 1,082 putative candidate monoallelic SNPs located in more than one hundred candidate genes. Among these, we found known imprinted genes, such as IPW, GRB10, INPP5F and ZNF597, which contribute to validate the approach. We also explored some likely candidates of our list and identified seven new imprinted genes, including ZFAT, ZFAT-AS1, GLIS3, NTM, MAGI2, ZC3H12Cand LIN28B, four of which encode zinc finger transcription factors. They are, however, not imprinted in the mouse placenta, except for Magi2. We analyzed in more details the ZFAT gene, which is paternally expressed in the placenta (as ZFAT-AS1, a non-coding antisense RNA) but biallelic in other tissues. The ZFAT protein is expressed in endothelial cells, as well as in syncytiotrophoblasts. The expression of this gene is, moreover, downregulated in placentas from complicated pregnancies. With this work we increase by about 10% the number of known imprinted genes in humans.
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影响因子：
5.3
作者：
Brideau, Chelsea M.;Eilertson, Kirsten E.;Soloway, Paul D.
通讯作者：
Soloway, Paul D.
影响因子：
4.5
作者：
Kanber D;Berulava T;Ammerpohl O;Mitter D;Richter J;Siebert R;Horsthemke B;Lohmann D;Buiting K
通讯作者：
Buiting K
影响因子：
64.8
作者：
CATTANACH, BM;KIRK, M
通讯作者：
KIRK, M
影响因子：
7
作者：
Choufani, Sanaa;Shapiro, Jonathan S.;Weksberg, Rosanna
通讯作者：
Weksberg, Rosanna
DOI：
10.1073/pnas.0308195101
发表时间：
2004-05-11
影响因子：
11.1
作者：
Li, J;Bench, AJ;Green, AR
通讯作者：
Green, AR