Exploring the role of two interacting phosphoinositide 3-kinases of Haemonchus contortus.
Exploring the role of two interacting phosphoinositide 3-kinases of Haemonchus contortus.
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探索捻转血矛线虫两种相互作用的磷酸肌醇 3-激酶的作用
DOI：
10.1186/s13071-014-0498-2
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发表时间：
2014-11-12
影响因子：
3.2
通讯作者：
Hu M
中科院分区：
文献类型：
--
作者：
Li FC;Gasser RB;Lok JB;Korhonen PK;Wang YF;Yin FY;He L;Zhou R;Zhao JL;Hu M
背景：磷酸肌肽3-激酶（PI3Ks）是一种相对保守的重要细胞内脂质激酶，参与信号传导和其他生物学途径。在自由生活的秀丽隐杆线虫中，PI3K的异二聚体形式由催化（AGE-1）和调节（AAP-1）亚基组成。这些亚基是胰岛素样信号通路的关键组成部分，在进入和退出水的调节中发挥作用。虽然，在寄生线虫中，类似的成分被提出来调节从自由生活阶段到寄生幼虫的过渡，但对于扭曲血蜱第三阶段幼虫（L3s）过渡到寄生的pi3k却一无所知。方法：采用综合分子方法研究秀丽隐杆线虫弯曲h的age-1和aap-1 （Hc-age-1和Hc-aap-1）。结果：Hc-age-1和Hc-aap-1两个基因在所有生命阶段均有转录，在卵、感染L3和成虫雌性中表达量最高。这些基因的表达定位于肠道，与秀丽隐杆线虫中同源基因的表达模式形成对比（它们在头部神经元和肠道中都表达）。酵母双杂交分析表明，Hc-AGE-1的接头结合域与Hc-AAP-1有较强的相互作用；然而，该复合体并没有挽救其同源物在age-1缺陷秀丽隐杆线虫中的功能。结论：这是首次从线虫中鉴定出pi3k编码基因。这一发现揭示了以Hc-age-1和Hc-aap-1为代表的PI3K异源二聚体在H. contortus发育生物学中的作用。
Background:Phosphoinositide 3-kinases (PI3Ks) are relatively conserved and important intracellular lipid kinases involved in signalling and other biological pathways. In the free-living nematode Caenorhabditis elegans, the heterodimeric form of PI3K consists of catalytic (AGE-1) and regulatory (AAP-1) subunits. These subunits are key components of the insulin-like signalling pathway and play roles in the regulation of the entry into and exit from dauer. Although, in parasitic nematodes, similar components are proposed to regulate the transition from free-living or arrested stages to parasitic larvae, nothing is known about PI3Ks in relation to the transition of third-stage larvae (L3s) to parasitism in Haemonchus contortus.Methods:An integrated molecular approach was used to investigate age-1 and aap-1 of H. contortus (Hc-age-1 and Hc-aap-1) in C. elegans.Results:The two genes Hc-age-1 and Hc-aap-1 were transcribed in all life stages, with the highest levels in the egg, infective L3 and adult female of H. contortus. The expression of these genes was localized to the intestine, contrasting the pattern of their orthologues in C. elegans (where they are expressed in both head neurons and the intestine). The yeast two-hybrid analysis demonstrated that the adaptor-binding domain of Hc-AGE-1 interacted strongly with the Hc-AAP-1; however, this complex did not rescue the function of its orthologue in age-1-deficient C. elegans.Conclusions:This is the first time that the PI3K-encoding genes have been characterized from a strongylid parasitic nematode. The findings provide insights into the role of the PI3K heterodimer represented by Hc-age-1 and Hc-aap-1 in the developmental biology of H. contortus.
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影响因子：
4.4
作者：
Cantacessi C;Campbell BE;Young ND;Jex AR;Hall RS;Presidente PJ;Zawadzki JL;Zhong W;Aleman-Meza B;Loukas A;Sternberg PW;Gasser RB
通讯作者：
Gasser RB
影响因子：
4
作者：
Hu M;Lok JB;Ranjit N;Massey HC Jr;Sternberg PW;Gasser RB
通讯作者：
Gasser RB
影响因子：
4
作者：
Brand, A;Hawdon, JM
通讯作者：
Hawdon, JM
影响因子：
4
作者：
Gao X;Frank D;Hawdon JM
通讯作者：
Hawdon JM
影响因子：
11.4
作者：
Hebert, DN;Foellmer, B;Helenius, A
通讯作者：
Helenius, A