Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus.
Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus.
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DOI：
10.1186/1471-2164-11-266
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发表时间：
2010-04-27
期刊：
影响因子：
4.4
通讯作者：
Gasser RB
中科院分区：
文献类型：
--
作者：
Cantacessi C;Campbell BE;Young ND;Jex AR;Hall RS;Presidente PJ;Zawadzki JL;Zhong W;Aleman-Meza B;Loukas A;Sternberg PW;Gasser RB
这种由缠绕血线虫引起的疾病在世界范围内具有重要的经济意义。这种胃线虫的感染性第三期幼虫(L3)被包裹在角质层(鞘)中，一旦被宿主与牧草一起摄取，就经历了标志着从自由生活(L3)到寄生(XL3)阶段的剥离过程。这项研究探索了与这种转变相关的基因转录的变化，并基于比较分析预测了关键转录本在与幼虫发育相关的代谢途径中的功能作用。用454测序技术测定了101,305(L3)和105,553(XL3)的表达序列标签(EST)，并对其进行了组装和注释；最丰富的转录本编码了类甲状腺激素、钙结合的EF-Hand、NAD(P)结合和核苷酸结合的蛋白以及钩虫分泌蛋白的同源物。在电子消减分析中，560和685个序列分别在L3和xL3阶段唯一表达；转录本编码核糖体蛋白、胶原蛋白和延伸因子(在L3中)，主要编码多肽酶和其他氨基酸分解代谢酶(在xL3中)。秀丽隐杆线虫在每个L3和xL3中唯一转录的转录本的同源基因被预测与所有其他基因相互作用，所有这些基因都参与了胚胎发育。目前的研究表明，在螺旋杆菌从L3到xL3阶段的转变过程中，发生了一些关键的转录变化，涉及与神经元组织(L3和xL3)的发育、角质层(L3)的形成和宿主血红蛋白(XL3)的消化有关的基因。未来使用下一代测序和生物信息学技术的努力将提供所需的效率和覆盖深度，以确定旋转毛虫不同发育阶段和/或组织的完整转录本以及这种重要寄生线虫的基因组。从应用的角度来看，这些进展应该会大大提高人们对扭曲血吸虫分子生物学的了解，并导致新的干预方法。
The disease caused by Haemonchus contortus, a blood-feeding nematode of small ruminants, is of major economic importance worldwide. The infective third-stage larva (L3) of this gastric nematode is enclosed in a cuticle (sheath) and, once ingested with herbage by the host, undergoes an exsheathment process that marks the transition from the free-living (L3) to the parasitic (xL3) stage. This study explored changes in gene transcription associated with this transition and predicted, based on comparative analysis, functional roles for key transcripts in the metabolic pathways linked to larval development. Totals of 101,305 (L3) and 105,553 (xL3) expressed sequence tags (ESTs) were determined using 454 sequencing technology, and then assembled and annotated; the most abundant transcripts encoded transthyretin-like, calcium-binding EF-hand, NAD(P)-binding and nucleotide-binding proteins as well as homologues of Ancylostoma-secreted proteins (ASPs). Using an in silico-subtractive analysis, 560 and 685 sequences were shown to be uniquely represented in the L3 and xL3 stages, respectively; the transcripts encoded ribosomal proteins, collagens and elongation factors (in L3), and mainly peptidases and other enzymes of amino acid catabolism (in xL3). Caenorhabditis elegans orthologues of transcripts that were uniquely transcribed in each L3 and xL3 were predicted to interact with a total of 535 other genes, all of which were involved in embryonic development. The present study indicated that some key transcriptional alterations taking place during the transition from the L3 to the xL3 stage of H. contortus involve genes predicted to be linked to the development of neuronal tissue (L3 and xL3), formation of the cuticle (L3) and digestion of host haemoglobin (xL3). Future efforts using next-generation sequencing and bioinformatic technologies should provide the efficiency and depth of coverage required for the determination of the complete transcriptomes of different developmental stages and/or tissues of H. contortus as well as the genome of this important parasitic nematode. Such advances should lead to a significantly improved understanding of the molecular biology of H. contortus and, from an applied perspective, to novel methods of intervention.
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影响因子：
16.2
作者：
Gomez, M;De Castro, E;Nef, P
通讯作者：
Nef, P
影响因子：
2.7
作者：
Estevez, Annette O.;Cowie, Robin H.;Estevez, Miguel
通讯作者：
Estevez, Miguel
影响因子：
1.3
作者：
Conder, GA;Johnson, SS
通讯作者：
Johnson, SS
DOI：
10.1073/pnas.91.6.2216
发表时间：
1994-03-15
影响因子：
11.1
作者：
BARNES, WM
通讯作者：
BARNES, WM
影响因子：
2.2
作者：
Bagnall, N.;Gough, J.;Kongsuwan, K.
通讯作者：
Kongsuwan, K.