COMT1 Silencing Aggravates Heat Stress-Induced Reduction in Photosynthesis by Decreasing Chlorophyll Content, Photosystem II Activity, and Electron Transport Efficiency in Tomato.
COMT1 Silencing Aggravates Heat Stress-Induced Reduction in Photosynthesis by Decreasing Chlorophyll Content, Photosystem II Activity, and Electron Transport Efficiency in Tomato.
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COMT1 沉默通过降低番茄中叶绿素含量、光系统 II 活性和电子传输效率来加剧热应激引起的光合作用减少
DOI：
10.3389/fpls.2018.00998
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发表时间：
2018
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Ahammed GJ;Xu W;Liu A;Chen S
尽管采取了一系列措施减少全球碳排放，但由于气候变化，全球平均气温正在上升。由于气温上升对粮食安全构成严重威胁，因此必须进一步探索能够赋予植物耐热性的重要生物分子。近年来，褪黑激素已成为一种普遍的非生物胁迫调节剂，可以提高植物对高温的耐受性。尽管如此，褪黑激素的这种调节作用主要是通过评估外源褪黑激素对植物对非生物胁迫的耐受性的影响来阐明的。在这里，我们产生褪黑激素缺乏番茄植物的褪黑激素生物合成基因，咖啡酸O-甲基转移酶1（COMT 1）的沉默，揭示内源褪黑激素在光合作用下的热应激的作用。我们研究了光合色素含量，叶片气体交换，和一系列的叶绿素荧光参数。结果表明，COMT 1基因的沉默通过抑制光合作用的光反应和碳固定反应加重了热胁迫。COMT 1基因沉默植株的光合色素含量、光吸收通量、能量耗散、光系统II（PSII）截面活性反应中心密度、光合电子传递速率、PSII光化学的最大光化学效率和CO2同化速率均低于非沉默植株，尤其是在高温胁迫下。然而，外源褪黑激素缓解热诱导的光合抑制在这两种基因型，表明褪黑激素是必要的压力条件下维持光合能力。这些发现为褪黑激素在光合作用中的重要作用提供了遗传学证据，因此可能对面对气候变化的园艺作物管理具有有益的启示。
Despite a range of initiatives to reduce global carbon emission, the mean global temperature is increasing due to climate change. Since rising temperatures pose a serious threat of food insecurity, it is important to further explore important biological molecules that can confer thermotolerance to plants. Recently, melatonin has emerged as a universal abiotic stress regulator that can enhance plant tolerance to high temperature. Nonetheless, such regulatory roles of melatonin were unraveled mainly by assessing the effect of exogenous melatonin on plant tolerance to abiotic stress. Here, we generated melatonin deficient tomato plants by silencing of a melatonin biosynthetic gene, CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), to unveil the role of endogenous melatonin in photosynthesis under heat stress. We examined photosynthetic pigment content, leaf gas exchange, and a range of chlorophyll fluorescence parameters. The results showed that silencing of COMT1 aggravated heat stress by inhibiting both the light reactions and the carbon fixation reactions of photosynthesis. The photosynthetic pigment content, light absorption flux, trapped energy flux, energy dissipation, density of active reaction center per photosystem II (PSII) cross-section, the photosynthetic electron transport rate, the maximum photochemical efficiency of PSII photochemistry, and the rate of CO2 assimilation all decreased in COMT1-silenced plants compared with that of non-silenced plants particularly under heat stress. However, exogenous melatonin alleviated heat-induced photosynthetic inhibition in both genotypes, indicating that melatonin is essential for maintaining photosynthetic capacity under stressful conditions. These findings provide genetic evidence on the vital role of melatonin in photosynthesis and thus may have useful implication in horticultural crop management in the face of climate change.
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影响因子：
5.6
作者：
Li H;Ahammed GJ;Zhou G;Xia X;Zhou J;Shi K;Yu J;Zhou Y
通讯作者：
Zhou Y
影响因子：
2.7
作者：
Rapacz, M.
通讯作者：
Rapacz, M.
DOI：
10.3390/molecules23010165
发表时间：
2018-01-15
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li X;Wei JP;Scott ER;Liu JW;Guo S;Li Y;Zhang L;Han WY
通讯作者：
Han WY
DOI：
10.3390/molecules23020386
发表时间：
2018-02-11
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Qi ZY;Wang KX;Yan MY;Kanwar MK;Li DY;Wijaya L;Alyemeni MN;Ahmad P;Zhou J
通讯作者：
Zhou J
影响因子：
10.3
作者：
Cai, Shu-Yu;Zhang, Yun;Zhou, Jie
通讯作者：
Zhou, Jie