Melatonin Alleviates High Temperature-Induced Pollen Abortion in Solanum lycopersicum.
Melatonin Alleviates High Temperature-Induced Pollen Abortion in Solanum lycopersicum.
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DOI：
10.3390/molecules23020386
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发表时间：
2018-02-11
期刊：
影响因子：
--
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Qi ZY;Wang KX;Yan MY;Kanwar MK;Li DY;Wijaya L;Alyemeni MN;Ahmad P;Zhou J
褪黑素是一种多效性信号分子，在调节植物生长发育以及对各种环境胁迫提供生理保护方面发挥着重要作用。尽管如此，褪黑激素介导的花粉耐热性的机制在很大程度上仍不清楚。在这项研究中，我们报道了用褪黑素(20µM)灌溉处理有效地改善了高温诱导的番茄(Solanum Lycpersicum)花粉失活和花粉萌发的抑制。褪黑素通过上调几种抗氧化酶的转录和活性来缓解高温下番茄花药中活性氧的产生。透射电子显微镜结果表明，高温诱导的花粉败育与单核小孢子早期绒毡层细胞过早退化和形成核退化的缺陷花粉粒有关，而褪黑素通过促进热休克蛋白基因的表达以重新折叠展开蛋白和自噬相关基因的表达以及形成自噬小体来降解变性蛋白来保护细胞器的退化。这些发现表明褪黑素具有保护高温下花粉活性的新功能，并支持褪黑素对植物生殖发育的潜在影响。
Melatonin is a pleiotropic signal molecule that plays critical roles in regulating plant growth and development, as well as providing physiological protections against various environmental stresses. Nonetheless, the mechanisms for melatonin-mediated pollen thermotolerance remain largely unknown. In this study, we report that irrigation treatment with melatonin (20 µM) effectively ameliorated high temperature-induced inactivation of pollen and inhibition of pollen germination in tomato (Solanum lycopersicum) plants. Melatonin alleviated reactive oxygen species production in tomato anthers under high temperature by the up-regulation of the transcription and activities of several antioxidant enzymes. Transmission electron micrograph results showed that high temperature-induced pollen abortion is associated with a premature degeneration of the tapetum cells and the formation of defective pollen grains with degenerated nuclei at the early uninuclear microspore stage, whilst melatonin protected degradation of organelles by enhancing the expression of heat shock protein genes to refold unfolded proteins and the expression of autophagy-related genes and formation of autophagosomes to degrade denatured proteins. These findings suggest a novel function of melatonin to protect pollen activity under high temperature and support the potential effects of melatonin on reproductive development of plants.
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影响因子：
6.9
作者：
Frank G;Pressman E;Ophir R;Althan L;Shaked R;Freedman M;Shen S;Firon N
通讯作者：
Firon N
影响因子：
--
作者：
Karapanos, I. C.;Akoumianakis, K. A.;Passam, Harold Christopher
通讯作者：
Passam, Harold Christopher
影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T
影响因子：
10.3
作者：
Lee, Kyungjin;Back, Kyoungwhan
通讯作者：
Back, Kyoungwhan
DOI：
10.1073/pnas.0608936104
发表时间：
2007-03-06
影响因子：
11.1
作者：
Francis, Kirk E.;Lam, Sandy Y.;Copenhaver, Gregory P.
通讯作者：
Copenhaver, Gregory P.