Natural variation in acyl editing is a determinant of seed storage oil composition.
Natural variation in acyl editing is a determinant of seed storage oil composition.
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DOI：
10.1038/s41598-018-35136-6
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发表时间：
2018-11-26
影响因子：
4.6
通讯作者：
Eastmond PJ
中科院分区：
文献类型：
--
作者：
Menard GN;Bryant FM;Kelly AA;Craddock CP;Lavagi I;Hassani-Pak K;Kurup S;Eastmond PJ
种子在其贮藏油的脂肪酸组成中表现出广泛的变化。然而，这种变异的遗传基础只被部分理解。本文利用多亲本高代互交（MAGIC）群体研究了拟南芥种子油脂肪酸链长度的遗传控制。我们绘制了四个数量性状基因座（QTL）的数量的主要非常长链脂肪酸物种11-二十碳烯酸（20：1），使用多个QTL建模。令人惊讶的是，主效应QTL与脂肪酸延长酶1不一致，并且平行的全基因组关联研究表明，溶血磷脂酰胆碱酰基转移酶2（LPCAT 2）是该QTL的候选者。回归分析还表明，LPCAT 2表达和20：1含量在19个MAGIC创始人种质的种子中相关。LPCAT是Lands循环的关键组分;一种酰基编辑途径，其使得酰基辅酶A和磷脂酰胆碱前体库之间的酰基交换分别用于微粒体脂肪酸延长和去饱和。我们使用双亲染色体片段替换系对主效QTL进行孟德尔分析，并进行互补试验，以表明LPCAT 2启动子中的单一顺式作用多态性通过改变LPCAT 2表达水平和发育角果中的总LPCAT活性而导致种子20：1含量的变化。我们的工作确定了油籽物种在酰基编辑的酶能力方面表现出自然变异，这有助于对储存油组成的遗传控制。
Seeds exhibit wide variation in the fatty acid composition of their storage oil. However, the genetic basis of this variation is only partially understood. Here we have used a multi-parent advanced generation inter-cross (MAGIC) population to study the genetic control of fatty acid chain length in Arabidopsis thaliana seed oil. We mapped four quantitative trait loci (QTL) for the quantity of the major very long chain fatty acid species 11-eicosenoic acid (20:1), using multiple QTL modelling. Surprisingly, the main-effect QTL does not coincide with FATTY ACID ELONGASE 1 and a parallel genome wide association study suggested that LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE 2 (LPCAT2) is a candidate for this QTL. Regression analysis also suggested that LPCAT2 expression and 20:1 content in seeds of the 19 MAGIC founder accessions are related. LPCAT is a key component of the Lands cycle; an acyl editing pathway that enables acyl-exchange between the acyl-Coenzyme A and phosphatidylcholine precursor pools used for microsomal fatty acid elongation and desaturation, respectively. We Mendelianised the main-effect QTL using biparental chromosome segment substitution lines and carried out complementation tests to show that a single cis-acting polymorphism in the LPCAT2 promoter causes the variation in seed 20:1 content, by altering the LPCAT2 expression level and total LPCAT activity in developing siliques. Our work establishes that oilseed species exhibit natural variation in the enzymic capacity for acyl editing and this contributes to the genetic control of storage oil composition.
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影响因子：
3.3
作者：
Imprialou M;Kahles A;Steffen JG;Osborne EJ;Gan X;Lempe J;Bhomra A;Belfield E;Visscher A;Greenhalgh R;Harberd NP;Goram R;Hein J;Robert-Seilaniantz A;Jones J;Stegle O;Kover P;Tsiantis M;Nordborg M;Rätsch G;Clark RM;Mott R
通讯作者：
Mott R
影响因子：
64.8
作者：
Gan X;Stegle O;Behr J;Steffen JG;Drewe P;Hildebrand KL;Lyngsoe R;Schultheiss SJ;Osborne EJ;Sreedharan VT;Kahles A;Bohnert R;Jean G;Derwent P;Kersey P;Belfield EJ;Harberd NP;Kemen E;Toomajian C;Kover PX;Clark RM;Rätsch G;Mott R
通讯作者：
Mott R
影响因子：
7.4
作者：
Hobbs, DH;Flintham, JE;Hills, MJ
通讯作者：
Hills, MJ
影响因子：
7.4
作者：
Bates, Philip D.;Fatihi, Abdelhak;Lu, Chaofu
通讯作者：
Lu, Chaofu
影响因子：
11.6
作者：
JAMES, DW;LIM, E;DOONER, HK
通讯作者：
DOONER, HK