Regulation by protein-tyrosine phosphatase PTP2 is distinct from that by PTP1 during Dictyostelium growth and development
Regulation by protein-tyrosine phosphatase PTP2 is distinct from that by PTP1 during Dictyostelium growth and development
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盘基网柄菌生长发育过程中蛋白酪氨酸磷酸酶 PTP2 的调节与 PTP1 的调节不同
DOI：
10.1128/mcb.14.8.5154-5164.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
R. Firtel
中科院分区：
文献类型：
--
作者：
Peter K. Howard;Marianne Gamper;Tony Hunter;R. Firtel
我们克隆了一个编码盘基网柄菌第二种蛋白--酪氨酸磷酸酶(PTP2)的基因，它的催化结构域与其他蛋白的氨基酸同源性约为30-39%，与盘基网柄菌PTP1的氨基酸同源性为41%。与PTP1一样，PTP2是一种非受体PTP，其催化结构域位于蛋白质的C端。PTP2的预测相对分子质量为43,000，在保守催化结构域的N端插入24个氨基酸，含58个氨基酸。PTP2转录本在营养细胞中中等水平表达，并在发育开始时被诱导数倍。利用PTP2-LacZ报告基因融合的研究表明，PTP2和PTP1一样，在多细胞发育阶段优先在茎前和前样细胞中表达。PTP2基因干扰物(ptp2缺失细胞)在生长过程中不会发生明显的变化，并且表现出与野生型细胞相似的时间发育模式。然而，ptp2缺失的鼻涕虫和子实体明显比野生型的大，这表明ptp2在调节多细胞结构中发挥了作用。从PTP2启动子过表达PTP2的盘状念珠菌菌株表现出生长速度和发育异常，其严重程度与PTP2过表达水平相对应。PTP2基因高表达的菌株在细菌草坪上生长缓慢，并在无菌条件下产生小细胞。当这些菌株开始发育时，细胞能够聚集，但随后停止进一步的形态发生6至8小时，在此之后，这些聚集的不同部分继续按正常时间进行，产生微小的子实体。PTP2的这些中断和过表达表型不同于相应的突变型PTP1表型。用抗磷酸酪氨酸抗体对生长发育过程中的PTP2突变菌株进行免疫检测发现，与野生型细胞相比，PTP2突变菌株的蛋白质酪氨酸磷酸化水平发生了一些变化。这些变化不同于先前鉴定的相应PTP1中断和过表达突变株中鉴定的那些变化。因此，虽然PTP2和PTP1是具有相似空间表达模式的非受体PTP，但我们的发现表明，它们在控制盘状芽孢杆菌的生长和发育方面具有不同的调节功能。
We have cloned a gene encoding a second Dictyostelium discoideum protein-tyrosine phosphatase (PTP2) whose catalytic domain has approximately 30 to 39% amino acid identity with those of other PTPs and a 41% amino acid identity with D. discoideum PTP1. Like PTP1, PTP2 is a nonreceptor PTP with the catalytic domain located at the C terminus of the protein. PTP2 has a predicted molecular weight of 43,000 and possesses an acidic 58-amino-acid insertion 24 amino acids from the N terminus of the conserved catalytic domain. PTP2 transcripts are expressed at moderate levels in vegetative cells and are induced severalfold at the onset of development. Studies with a PTP2-lacZ reporter gene fusion indicate that PTP2, like PTP1, is preferentially expressed in prestalk and anterior-like cell types during the multicellular stages of development. PTP2 gene disruptants (ptp2 null cells) are not detectably altered in growth and show a temporal pattern of development similar to that of wild-type cells. ptp2 null slugs and fruiting bodies, however, are significantly larger than those of wild-type slugs, suggesting a role for PTP2 in regulating multicellular structures. D. discoideum strains overexpressing PTP2 from the PTP2 promoter exhibit growth rate and developmental abnormalities, the severity of which corresponds to the level of PTP2 overexpression. Strains with high overexpression of the PTP2 gene grow slowly on bacterial lawns and produce small cells in axenic medium. When development is initiated in these strains, cells are able to aggregate but then stop further morphogenesis for 6 to 8 h, after which time a variable fraction of these aggregates continue with normal timing, producing diminutive fruiting bodies. These disruption and overexpression phenotypes for PTP2 are distinct from the corresponding mutant PTP1 phenotypes. Immunoprobing PTP2 mutant strains during growth and development with antiphosphotyrosine antibodies reveals several changes in the tyrosine phosphorylation of proteins in PTP2 mutant strains compared with that in wild-type cells. These changes are different from those identified in the previously characterized corresponding PTP1 disruption and overexpression mutant strains. Thus, although PTP2 and PTP1 are nonreceptor PTPs with similar spatial patterns of expression, our findings suggest that they possess distinct regulatory functions in controlling D. discoideum growth and development.
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盘基网柄菌的分子生物学：工具和应用。
影响因子：
--
作者：
Nellen,W;Datta,S;Reymond,C;Sivertsen,A;Mann,S;Crowley,T;Firtel,RA
通讯作者：
Firtel,RA
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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通讯作者：
Trowbridge,IS
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影响因子：
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作者：
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通讯作者：
Sefton,BM
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DOI：
10.1073/pnas.88.8.3455
发表时间：
1991
影响因子：
11.1
作者：
Ottilie,S;Chernoff,J;Hannig,G;Hoffman,CS;Erikson,RL
通讯作者：
Erikson,RL