Incubation of murine bone marrow cells in hypoxia ensures the maintenance of marrow‐repopulating ability together with the expansion of committed progenitors
Incubation of murine bone marrow cells in hypoxia ensures the maintenance of marrow‐repopulating ability together with the expansion of committed progenitors
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在缺氧条件下孵育小鼠骨髓细胞可确保维持骨髓再生能力以及定向祖细胞的扩增
DOI：
10.1046/j.1365-2141.2000.01842.x
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发表时间：
2000
影响因子：
6.5
通讯作者：
P. D. Sbarba
中科院分区：
文献类型：
--
作者：
Zoran Ivanovic;B. Bartolozzi;Pietro Antonio Bernabei;M. G. Cipolleschi;E. Rovida;Pavle Milenković;V. Praloran;P. D. Sbarba
我们以前开发了一种低氧培养系统，在该系统中，具有骨髓再生能力(MRA)的祖细胞与承诺的祖细胞不同，被选择并保持得比在空气中更好。我们在此报告对这一系统的改进，旨在将维持MRA的祖细胞的维护与多能和承诺的祖细胞的数量扩展相结合。小鼠骨髓细胞在含干细胞因子、粒细胞集落刺激因子、白介素6和白介素3的液体培养液中于1%氧气中孵育。在低氧培养第8天，高增殖能力细胞和粒/巨噬细胞集落形成细胞(HPP-CFC和CFU-GM)的数量随着时间的推移而增加。来自低氧培养物的HPP-CFC产生的集落具有较高的复制能力，而来自对照培养物的HPP-CFC产生的集落表现出较低的复制能力。MRA在低氧条件下完全保持，在空气中明显降低。因此，严重缺氧能够确保维持MRA的祖细胞完全维持，同时显著扩大体外可检测的克隆性祖细胞，包括那些被赋予复制能力的克隆祖细胞。该系统有助于改进现有的体外处理移植前人类造血细胞群体的技术。
We developed previously a hypoxic culture system in which progenitors endowed with marrow‐repopulating ability (MRA), unlike committed progenitors, were selected and maintained better than in air. We report here an improvement to this system targeted at combining the maintenance of progenitors sustaining MRA with the numerical expansion of multipotent and committed progenitors. Murine bone marrow cells were incubated at 1% oxygen in liquid medium supplemented with stem cell factor, granulocyte colony‐stimulating factor, interleukin‐6 and interleukin‐3. In day 8 hypoxic cultures, the numbers of high proliferative potential and granulocyte/macrophage colony‐forming cells (HPP‐CFC and CFU‐GM) were increased with respect to time zero. Colonies generated by HPP‐CFC derived from hypoxic cultures exhibited a high replating ability, whereas colonies generated by HPP‐CFC derived from control cultures exhibited a low replating ability. MRA was fully maintained in hypoxia and markedly reduced in air. Thus, severe hypoxia is able to ensure a full maintenance of progenitors sustaining MRA, together with a significant expansion of in vitro‐detectable clonogenic progenitors, including those endowed with replating ability. This system could contribute to the improvement of current techniques for the in vitro treatment of human haematopoietic cell populations before transplantation.
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使用白细胞介素 3 (IL-3)、IL-6、IL-11 和干细胞因子对小鼠骨髓细胞进行离体扩增会导致在受辐射宿主中的植入受损。
影响因子：
20.3
作者：
Peters,SO;Kittler,EL;Ramshaw,HS;Quesenberry,PJ
通讯作者：
Quesenberry,PJ
影响因子：
56.9
作者：
BERARDI, AC;WANG, AL;SCADDEN, DT
通讯作者：
SCADDEN, DT
增殖诱导的原始造血祖细胞活性下降与离体扩增的 CD34 细胞凋亡增加相结合。
DOI：
--
发表时间：
1998
期刊：
Experimental hematology.
影响因子：
--
作者：
Traycoff,CM;Orazi,A;Ladd,AC;Rice,S;McMahel,J;Srour,EF
通讯作者：
Srour,EF
氧张力改变细胞因子对巨核细胞、红细胞和粒细胞谱系的影响。
DOI：
--
发表时间：
1998
期刊：
Experimental hematology.
影响因子：
--
作者：
LaIuppa,JA;Papoutsakis,ET;Miller,WM
通讯作者：
Miller,WM