Investigating the Swimming of Microbial Pathogens Using Digital Holography.

Investigating the Swimming of Microbial Pathogens Using Digital Holography.
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使用数字全息术研究微生物病原体的游动。

DOI:
10.1007/978-3-319-32189-9_3
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发表时间:
2016
影响因子:
--
通讯作者:
Thornton KL
Thornton KL
中科院分区:
医学4区
文献类型:
--
作者:
Thornton KL

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为了了解微生物病原体的大部分行为,有必要对活细胞、它们之间的相互作用以及与宿主细胞的相互作用进行成像。像大肠杆菌这样的物种很难成像:它们通常是显微镜下的,无色透明。传统的细胞可视化技术,如荧光标记或相衬显微镜,可以提供二维细胞行为的良好信息,但不能提供三维细胞运动的信息。我们回顾了数字全息显微镜在高速三维成像中的应用,并展示了其用于捕获三种重要模型病原体的形状和游动行为:E。杆菌、Plasmodiumspp。andLeishmaniaspp。
To understand much of the behaviour of microbial pathogens, it is necessary to image living cells, their interactions with each other and with host cells. Species such asEscherichia coliare difficult subjects to image: they are typically microscopic, colourless and transparent. Traditional cell visualisation techniques such as fluorescent tagging or phase-contrast microscopy give excellent information on cell behaviour in two dimensions, but no information about cells moving in three dimensions. We review the use of digital holographic microscopy for three-dimensional imaging at high speeds, and demonstrate its use for capturing the shape and swimming behaviour of three important model pathogens:E. coli,Plasmodiumspp. andLeishmaniaspp.
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