Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131.
Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131.
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DOI：
10.1101/gr.216606.116
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发表时间：
2017-08-01
期刊：
影响因子：
7
通讯作者：
Parkhill J
中科院分区：
文献类型：
--
作者：
Kallonen T;Brodrick HJ;Harris SR;Corander J;Brown NM;Martin V;Peacock SJ;Parkhill J
与尿路感染和菌血症相关的大肠杆菌已被深入研究，包括最近的工作重点是毒性，全球传播，多药耐药谱系ST 131。将ST 131置于更广泛的E.大肠杆菌群体与疾病相关，我们使用基因组学分析了一个系统的11年的医院为基础的调查，使用由英国抗微生物化疗学会和剑桥大学医院NHS基金会信托从英格兰各地收集的分离株，对最成功的谱系的种群动态分析确定了ST 131和ST 69的出现及其建立，它们是与ST 73、ST 95和ST 12一起沿着的五个最常见的谱系中的两个。最常鉴定的谱系是ST 73。与ST 131相比，ST 73对大多数抗生素敏感，表明多重耐药不是大肠埃希菌流行的主要原因。大肠杆菌谱系在这个群体中。ST 69和ST 131出现的时间系统发育分析确定了出现的动态差异，并表明ST 131在这个群体中的扩展不是由连续出现的抗性越来越强的亚枝驱动的。我们发现，随着时间的推移，E。大肠杆菌种群在迅速达到新的平衡之前，只是被新谱系的引入短暂地扰乱。总之，这些发现表明，E。侵袭性疾病中的大肠杆菌谱系是由医院外发生的负频率依赖性选择驱动的，最有可能是在共生生态位中，并且耐药性不是该生态位成功的主要决定因素。
Escherichia coli associated with urinary tract infections and bacteremia has been intensively investigated, including recent work focusing on the virulent, globally disseminated, multidrug-resistant lineage ST131. To contextualize ST131 within the broader E. coli population associated with disease, we used genomics to analyze a systematic 11-yr hospital-based survey of E. coli associated with bacteremia using isolates collected from across England by the British Society for Antimicrobial Chemotherapy and from the Cambridge University Hospitals NHS Foundation Trust. Population dynamics analysis of the most successful lineages identified the emergence of ST131 and ST69 and their establishment as two of the five most common lineages along with ST73, ST95, and ST12. The most frequently identified lineage was ST73. Compared to ST131, ST73 was susceptible to most antibiotics, indicating that multidrug resistance was not the dominant reason for prevalence of E. coli lineages in this population. Temporal phylogenetic analysis of the emergence of ST69 and ST131 identified differences in the dynamics of emergence and showed that expansion of ST131 in this population was not driven by sequential emergence of increasingly resistant subclades. We showed that over time, the E. coli population was only transiently disturbed by the introduction of new lineages before a new equilibrium was rapidly achieved. Together, these findings suggest that the frequency of E. coli lineages in invasive disease is driven by negative frequency-dependent selection occurring outside of the hospital, most probably in the commensal niche, and that drug resistance is not a primary determinant of success in this niche.
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影响因子：
5.2
作者：
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通讯作者：
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影响因子：
3.1
作者：
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影响因子：
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作者：
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通讯作者：
Beatson SA
DOI：
10.1073/pnas.1522510113
发表时间：
2016-03-29
影响因子：
11.1
作者：
Chatzidaki-Livanis, Maria;Geva-Zatorsky, Naama;Comstock, Laurie E.
通讯作者：
Comstock, Laurie E.
影响因子：
5.2
作者：
Andrews, JM
通讯作者：
Andrews, JM