Effects of GLP-1 Infusion Upon Whole-body Glucose Uptake and Skeletal Muscle Perfusion During Fed-state in Older Men.
Effects of GLP-1 Infusion Upon Whole-body Glucose Uptake and Skeletal Muscle Perfusion During Fed-state in Older Men.
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GLP-1输注对老年男性进食状态下全身葡萄糖摄取和骨骼肌灌注的影响。
DOI：
10.1210/clinem/dgac613
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发表时间：
2023-03-10
期刊：
影响因子：
--
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老化的骨骼肌变得胰岛素抵抗和萎缩。激素胰高血糖素样肽1 （GLP-1）促进餐后葡萄糖摄取并增加肌肉灌注，独立于胰岛素的作用。因此，我们假设外源性GLP-1输注可以增强肌肉灌注，并对老年人进食状态钳夹期间的葡萄糖代谢产生积极影响。在一项随机交叉试验中研究了8名男性（71±1岁）。在餐后（进食状态）胰岛素和葡萄糖钳夹前采集基础血液样本，并辅以氨基酸输注，持续3小时。有鉴于此，在外周和股血管插管和基线测量后，外周静脉输注奥曲肽、胰岛素（Actrapid）、20%葡萄糖和混合氨基酸；开始或不进行股动脉GLP-1输注的Vamin 14-EF。ELISA法检测GLP-1、胰岛素、c肽水平。通过超声造影评估肌肉微血管血流。通过葡萄糖输注速率参数测定全身葡萄糖处理。与单独喂养相比，GLP-1显著增加了骨骼肌微血管血流量（分别是基础的5.0±2.1和1.9±0.7倍，P = 0.008），同时也增加了全身葡萄糖摄取（曲线下面积16.9±1.7 vs 11.4±1.8 mg/kg -1 /180分钟-1，P = 0.02±GLP）。GLP-1对全身血糖控制的有益作用是明显的，胰岛素被固定在喂食状态的水平。GLP-1进一步增强了胰岛素对老年男性全身葡萄糖摄取的影响，强调了其作为治疗靶点的作用。GLP-1增强微血管流动的作用可能也影响其他葡萄糖调节器官，反映在更大的全身葡萄糖摄取上。
Ageing skeletal muscles become both insulin resistant and atrophic. The hormone glucagon-like peptide 1 (GLP-1) facilitates postprandial glucose uptake as well as augmenting muscle perfusion, independent of insulin action. We thus hypothesized exogenous GLP-1 infusions would enhance muscle perfusion and positively affect glucose metabolism during fed-state clamps in older people. Eight men (71 ± 1 years) were studied in a randomized crossover trial. Basal blood samples were taken before postprandial (fed-state) insulin and glucose clamps, accompanied by amino acid infusions, for 3 hours. Reflecting this, following insertions of peripheral and femoral vessels cannulae and baseline measurements, peripheral IV infusions of octreotide, insulin (Actrapid), 20% glucose, and mixed amino acids; Vamin 14-EF with or without a femoral arterial GLP-1 infusion were started. GLP-1, insulin, and C-peptide were measured by ELISA. Muscle microvascular blood flow was assessed via contrast enhanced ultrasound. Whole-body glucose handling was assayed by assessing glucose infusion rate parameters. Skeletal muscle microvascular blood flow significantly increased in response to GLP-1 vs feeding alone (5.0 ± 2.1 vs 1.9 ± 0.7 fold-change from basal, respectively; P = 0.008), while also increasing whole-body glucose uptake (area under the curve 16.9 ± 1.7 vs 11.4 ± 1.8 mg/kg−1/180 minutes−1, P = 0.02 ± GLP, respectively). The beneficial effects of GLP-1 on whole-body glycemic control are evident with insulin clamped at fed-state levels. GLP-1 further enhances the effects of insulin on whole-body glucose uptake in older men, underlining its role as a therapeutic target. The effects of GLP-1 in enhancing microvascular flow likely also affects other glucose-regulatory organs, reflected by greater whole-body glucose uptake.
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DOI：
10.1093/gerona/glr036
发表时间：
2011-06-01
影响因子：
5.1
作者：
Chevalier, Stephanie;Goulet, Eric D. B.;Morais, Jose A.
通讯作者：
Morais, Jose A.
氨基酸和胰岛素对人类肌肉中信号传导，泛素连接酶和蛋白质更新的作用之间的分离。
DOI：
10.1152/ajpendo.90411.2008
发表时间：
2008-09
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
Greenhaff PL;Karagounis LG;Peirce N;Simpson EJ;Hazell M;Layfield R;Wackerhage H;Smith K;Atherton P;Selby A;Rennie MJ
通讯作者：
Rennie MJ
影响因子：
8.3
作者：
Hamilton, CA;Brosnan, MJ;Dominiczak, AF
通讯作者：
Dominiczak, AF
血管紧张素 II 1 型和 2 型受体调节基底骨骼肌微血管容量和葡萄糖使用
影响因子：
8.3
作者：
Chai, Weidong;Wang, Wenhui;Liu, Zhenqi
通讯作者：
Liu, Zhenqi
影响因子：
158.5
作者：
GUTNIAK, M;ORSKOV, C;EFENDIC, S
通讯作者：
EFENDIC, S