Genomic Analysis of the Evolution and Global Spread of Hyper-invasive Meningococcal Lineage 5.
Genomic Analysis of the Evolution and Global Spread of Hyper-invasive Meningococcal Lineage 5.
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高侵入性脑膜炎球菌谱系的进化和全局扩散的基因组分析5。
DOI：
10.1016/j.ebiom.2015.01.004
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发表时间：
2015-03-01
期刊：
影响因子：
11.1
通讯作者：
Caugant, Dominique A.
中科院分区：
文献类型：
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作者：
Harrison, Odile B.;Bray, James E.;Maiden, Martin C. J.;Caugant, Dominique A.
细菌大流行的主要模式是出现一种致命的变种，随着它在人群中的传播而多样化。我们调查了一个40年的脑膜炎球菌病大流行引起的超侵入性ET-5/ST-32复合物。在http://pubmlst.org/neisseria上对1969年至2008年全球收集的脑膜炎奈瑟氏球菌分离株进行了全基因组测序（WGS），并使用逐基因方法进行了分析。WGS数据的分析鉴定了1940个基因的“谱系5泛基因组”，其中1752个（92%）存在于所有分离株中（谱系5“核心基因组”）。遗传多样性，这主要是由水平基因转移产生的，是不均匀分布在基因组中，然而，不同的和保守的核心基因，辅助基因，抗原编码基因的系谱分析，鲁棒地确定了一个星星与一些亚系的同源性。大多数欧洲和美国分离株属于两个密切相关的亚系之一，这两个亚系在20世纪70年代确定大流行之前已经多样化。第三个，遗传上更多样化的亚系，与亚洲分离株有关。几个分离株获得了相关淋球菌的DNA。这些数据与单一起源点随后大流行传播不一致，而是表明亚系已经多样化并通过无症状传播，多种不同的菌株导致局部高流行性疫情。超侵入性N.脑膜炎谱系5区分亚谱系和局部暴发。淋球菌接合性质粒的鉴定与水平遗传转移一致。在奈瑟氏菌中发现一种以前未鉴定的独特的4型分泌系统。对保守和多样基因座的逐基因分析为血清群B疫苗开发提供了必要工具。
The predominant model for bacterial pandemics is the emergence of a virulent variant that diversifies as it spreads in human populations. We investigated a 40-year meningococcal disease pandemic caused by the hyper-invasive ET-5/ST-32 complex. A global collection of Neisseria meningitidis isolates dating from 1969 to 2008 was whole genome sequenced (WGS) and analysed using a gene-by-gene approach at http://pubmlst.org/neisseria. Analysis of WGS data identified a ‘Lineage 5 pan genome’ of 1940 genes, 1752 (92%) of which were present in all isolates (Lineage 5 ‘core genome’). Genetic diversity, which was mostly generated by horizontal gene transfer, was unevenly distributed in the genome; however, genealogical analysis of diverse and conserved core genes, accessory genes, and antigen encoding genes, robustly identified a star phylogeny with a number of sub-lineages. Most European and American isolates belonged to one of two closely related sub-lineages, which had diversified before the identification of the pandemic in the 1970s. A third, genetically more diverse sub-lineage, was associated with Asian isolates. Several isolates had acquired DNA from the related gonococcus. These data were inconsistent with a single point of origin followed by pandemic spread, rather suggesting that the sub-lineages had diversified and spread by asymptomatic transmission, with multiple distinct strains causing localised hyperendemic outbreaks. Genomic analysis of hyper-invasive N. meningitidis lineage 5 distinguishing sub-lineages and localised outbreaks. Identification of a gonococcal conjugative plasmid consistent with horizontal genetic transfer. Discovery of a distinct type 4 secretion system previously unidentified in Neisseria. Gene-by-gene analysis of conserved and diverse loci providing essential tools in serogroup B vaccine development.
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影响因子：
10.7
作者：
Bryant, D;Moulton, V
通讯作者：
Moulton, V
影响因子：
9.5
作者：
Milne, Iain;Stephen, Gordon;Marshall, David
通讯作者：
Marshall, David
DOI：
10.1073/pnas.0712019105
发表时间：
2008-09-30
影响因子：
11.1
作者：
Buckee, Caroline O.;Jolley, Keith A.;Maiden, Martin C. J.
通讯作者：
Maiden, Martin C. J.
影响因子：
3.7
作者：
Pachulec, Emilia;van der Does, Chris
通讯作者：
van der Does, Chris
DOI：
10.1007/bf00415492
发表时间：
1987-01-01
期刊：
ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY
影响因子：
--
作者：
CAUGANT, DA;FROHOLM, LO;SELANDER, RK
通讯作者：
SELANDER, RK