Surfactant inhibits cationic liposome-mediated gene transfer.
Surfactant inhibits cationic liposome-mediated gene transfer.
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表面活性剂抑制阳离子脂质体介导的基因转移。
DOI：
10.1089/hum.1997.8.7-817
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发表时间：
1997
影响因子：
4.2
通讯作者：
J. White
中科院分区：
文献类型：
--
作者：
M. Tsan;G. L. Tsan;J. White
我们已经证明，气管吹入重组质粒DNA的结果在大鼠肺的转染到相同的程度吹入质粒-阳离子脂质体复合物。为了更好地理解这一观察结果，我们研究了阳离子脂质体存在和不存在下质粒DNA的体外基因转移以及表面活性剂对基因转移的影响。用氯霉素乙酰转移酶（CAT）表达质粒pBL-CAT和pSV-CAT在大鼠胎肺成纤维细胞（RFL-6）、小牛肺动脉内皮细胞（CPAE）和大鼠II型肺泡上皮细胞（II型AE）中进行表达。测试了三种阳离子脂质体：DDAB（二甲基-双十八烷基溴化铵）-脂质体、DOTAP（二油酰三甲基铵丙烷）-脂质体和脂质体转染素。结果显示，（i）单独的质粒DNA引起剂量依赖性的低水平转染，在RFL-6中最有效，其次是CPAE和II型AE，（ii）DDAB-脂质体显著增强基因转移，在RFL-6中最有效，其次是CPAE和II型AE，（iii）Survanta，一种天然衍生的表面活性剂制剂，和Exosurf，一种合成表面活性剂，虽然对单独质粒DNA的体外基因转移没有影响，但显著抑制阳离子脂质体介导的基因转移，（iv）二棕榈酰磷脂酰胆碱负责Exosurf的抑制作用，Exosurf对阳离子脂质体介导的基因转移的抑制不是由于抑制质粒DNA-阳离子脂质体复合物的摄取或干扰启动子和增强子。所观察到的表面活性剂对阳离子脂质体介导的基因转移的抑制可以部分解释我们先前的观察结果，即质粒DNA和质粒-阳离子脂质体复合物的气管吹入导致相等的肺基因转移。
We have demonstrated that tracheal insufflation of recombinant plasmid DNA results in transfection of rat lungs to the same extent as insufflation of plasmid-cationic liposome complex. To understand this observation better, we investigated the in vitro gene transfer of plasmid DNA in the presence and absence of cationic liposome and the effect of surfactant on gene transfer. The chloramphenicol acetyltransferase (CAT) expression plasmids pBL-CAT and pSV-CAT were studied in three cell types: rat fetal lung fibroblast (RFL-6), calf pulmonary artery endothelial cell (CPAE), and rat type II alveolar epithelial cell (type II AE). Three cationic liposomes were tested: DDAB (dimethyl-dioctadecyl ammonium bromide)-liposome, DOTAP (dioleoyltrimethyl ammonium propane)-liposome, and lipofectin. The results revealed that (i) plasmid DNA alone caused a dose-dependent, low-level transfection, most efficiently in RFL-6 followed by CPAE and type II AE, (ii) DDAB-liposome markedly enhanced gene transfer, most efficiently in RFL-6 followed by CPAE and type II AE, (iii) Survanta, a naturally derived surfactant preparation, and Exosurf, a synthetic surfactant, while having no effect on in vitro gene transfer by plasmid DNA alone, markedly inhibited cationic liposome-mediated gene transfer, (iv) dipalmitoyl phosphatidylcholine was responsible for the inhibitory effect of Exosurf, and (v) inhibition of cationic liposome-mediated gene transfer by Exosurf was not due to inhibition of plasmid DNA-cationic liposome complex uptake or interference with the promoter and enhancer. The observed inhibition of cationic liposome-mediated gene transfer by surfactant may in part explain our previous observation that tracheal insufflation of plasmid DNA and plasmid-cationic liposome complex results in equal lung gene transfer.
DOI：
10.1165/ajrcmb/8.5.556
发表时间：
1993-05
影响因子：
6.4
作者：
D. Froh;L. Gonzales;P. L. Ballard
通讯作者：
D. Froh;L. Gonzales;P. L. Ballard
影响因子：
4.2
作者：
HICKMAN, MA;MALONE, RW;POWELL, JS
通讯作者：
POWELL, JS
DOI：
10.1073/pnas.84.21.7413
发表时间：
1987-11-01
影响因子：
11.1
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
通讯作者：
DANIELSEN, M