Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system.
Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system.
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DOI：
10.1007/s10544-012-9718-8
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发表时间：
2013-08
影响因子：
2.8
通讯作者：
Zhang, John X. J.
中科院分区：
文献类型：
--
作者：
Huang, Yu-yen;Hoshino, Kazunori;Chen, Peng;Wu, Chun-hsien;Lane, Nancy;Huebschman, Michael;Liu, Huaying;Sokolov, Konstantin;Uhr, Jonathan W.;Frenkel, Eugene P.;Zhang, John X. J.
关键词：
结合免疫磁分析和微流控微芯片操作的力量，我们成功地从临床血液样本中检测到罕见的ctc。微流控系统以触发器模式操作，其中计算机控制的带有微流控芯片阵列的旋转支架反转微通道。我们已经从理论上和实验上证明，红细胞（RBC）的沉降方向与细胞分离所需的磁力有关，对捕获效率、吞吐量和纯度都很重要。触发器操作通过改变磁场相对于重力的方向，减少了红细胞的停滞和捕获表面上的非特异性结合。开发的基于免疫磁性微芯片的筛选系统在加标筛选实验中对SkBr3、PC3和Colo205细胞系显示出高捕获率（超过90%），并成功地从患者血液样本中分离出ctc。提出的运动控制微芯片免疫磁系统作为癌症诊断和预后的临床工具有很大的前景。
Combining the power of immunomagnetic assay and microfluidic microchip operations, we successfully detected rare CTCs from clinical blood samples. The microfluidic system is operated in a flip-flop mode, where a computer-controlled rotational holder with an array of microfluidic chips inverts the microchannels. We have demonstrated both theoretically and experimentally that the direction of red blood cell (RBC) sedimentation with regards to the magnetic force required for cell separation is important for capture efficiency, throughput, and purity. The flip-flop operation reduces the stagnation of RBCs and non-specific binding on the capture surface by alternating the direction of the magnetic field with respect to gravity. The developed immunomagnetic microchip-based screening system exhibits high capture rates (more than 90%) for SkBr3, PC3, and Colo205 cell lines in spiked screening experiments and successfully isolates CTCs from patient blood samples. The proposed motion controlled microchip-based immunomagnetic system shows great promise as a clinical tool for cancer diagnosis and prognosis.
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影响因子：
6.1
作者：
Kang, Joo H.;Krause, Silva;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
影响因子：
2.3
作者：
Sieben, V. J.;Marun, C. S. Debes;Backhouse, C. J.
通讯作者：
Backhouse, C. J.
影响因子：
6.1
作者：
Yung, Chong Wing;Fiering, Jason;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
DOI：
10.1073/pnas.1012539107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Stott, Shannon L.;Hsu, Chia-Hsien;Toner, Mehmet
通讯作者：
Toner, Mehmet
DOI：
10.1158/1078-0432.ccr-08-2036
发表时间：
2009-03-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Leversha MA;Han J;Asgari Z;Danila DC;Lin O;Gonzalez-Espinoza R;Anand A;Lilja H;Heller G;Fleisher M;Scher HI
通讯作者：
Scher HI