Establishment of human patient-derived endometrial cancer xenografts in NOD scid gamma mice for the study of invasion and metastasis.
Establishment of human patient-derived endometrial cancer xenografts in NOD scid gamma mice for the study of invasion and metastasis.
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DOI：
10.1371/journal.pone.0116064
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kim JJ
中科院分区：
文献类型：
--
作者：
Unno K;Ono M;Winder AD;Maniar KP;Paintal AS;Yu Y;Wei JJ;Lurain JR;Kim JJ
Most endometrial cancers are detected early and have a good prognosis, while some endometrial cancers are highly invasive, metastasize early, and respond suboptimally to therapy. Currently, appropriate model systems to study the aggressive nature of these tumors are lacking. The objective of this study was to establish a mouse xenograft model of endometrial tumors derived from patients in order to study the biological aggressive characteristics that underlie invasion and metastasis. Endometrial tumor tissue fragments (1.5 mm×1.5 mm) from patients undergoing surgery, were transplanted under the renal capsule of NOD scid gamma mice. After 6–8 weeks, tumors were excised and serially transplanted into additional mice for propagation. Immunohistochemical analysis of the tumors was done for various tumor markers. Four cases of different subtypes of endometrial cancer were grown and propagated in mice. Three of the four tumor cases invaded into the kidneys and to adjacent organs. While all tumors exhibited minimal to no staining for estrogen receptor α, progesterone receptor staining was observed for tumor grafts. In addition, levels and localization of E-cadherin, cytokeratin and vimentin varied depending on subtype. Finally, all tumor xenografts stained positively for urokinase plasminogen activator while 3 tumor xenografts, which showed invasive characteristics, stained positively for urokinase plasminogen activator receptor. Endometrial tumors transplanted under the renal capsule exhibit growth, invasion and local spread. These tumors can be propagated and used to study aggressive endometrial cancer.
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影响因子：
64.8
作者：
通讯作者：
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影响因子：
4.7
作者：
Press, Joshua Z.;Kenyon, Jennifer A.;Wang, Y. Z.
通讯作者：
Wang, Y. Z.
影响因子：
5.6
作者：
HENDRICKSON, M;ROSS, J;KEMPSON, R
通讯作者：
KEMPSON, R
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3.7
作者：
Ono M;Qiang W;Serna VA;Yin P;Coon JS 5th;Navarro A;Monsivais D;Kakinuma T;Dyson M;Druschitz S;Unno K;Kurita T;Bulun SE
通讯作者：
Bulun SE
影响因子：
254.7
作者：
Siegel, Rebecca;Naishadham, Deepa;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin