Stabilization of SARS-CoV-2 RNA in wastewater via rapid RNA extraction.
Stabilization of SARS-CoV-2 RNA in wastewater via rapid RNA extraction.
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DOI：
10.1016/j.scitotenv.2023.162992
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发表时间：
2023-06-20
影响因子：
9.8
通讯作者：
Berry, Scott M.
中科院分区：
文献类型：
--
作者：
Torabi, Soroosh;Amirsoleimani, Atena;Banadaki, Mohammad Dehghan;Strike, William Dalton;Rockward, Alexus;Noble, Ann;Liversedge, Matthew;Keck, James W.;Berry, Scott M.
基于废水的流行病学（WBE）为世界各地社区的SARS-CoV-2监测做出了贡献。有症状和无症状的COVID-19患者都可以通过胃肠道排出病毒，从而可以量化粪便中的病毒并最终量化废水（WW）。不幸的是，废水中SARS-CoV-2 RNA的不稳定性限制了WBE计划的效用，特别是在可能无法获得可靠冷藏和/或快速运输的偏远/农村地区。本研究考察了在样品采集当天快速提取SARS-CoV-2 RNA是否可以最大限度地减少降解。重要的是，在这些实验中使用的萃取技术，称为基于排除的样品制备（ESP），重量轻，便携，无电，使其适合在远程环境中实施。我们证明，与在4°C或环境温度下储存的原始废水样品相比，立即提取RNA然后进行环境储存显着增加了RNA半衰期。鉴于RNA降解对WBE测量的灵敏度和精度都有负面影响，必须努力减轻降解，以最大限度地发挥WBE对公共卫生的潜在影响。
Wastewater-based Epidemiology (WBE) has contributed to surveillance of SARS-CoV-2 in communities across the world. Both symptomatic and asymptomatic patients with COVID-19 can shed the virus through the gastrointestinal tract, enabling the quantification of the virus in stool and ultimately in wastewater (WW). Unfortunately, instability of SARS-CoV-2 RNA in wastewater limits the utility of WBE programs, particularly in remote/rural regions where reliable cold storage and/or rapid shipping may be unavailable. This study examined whether rapid SARS-CoV-2 RNA extraction on the day of sample collection could minimize degradation. Importantly, the extraction technology used in these experiments, termed exclusion-based sample preparation (ESP), is lightweight, portable, and electricity-free, making it suitable for implementation in remote settings. We demonstrated that immediate RNA extraction followed by ambient storage significantly increased the RNA half-life compared to raw wastewater samples stored at both 4 °C or ambient temperature. Given that RNA degradation negatively impacts both the sensitivity and precision of WBE measurements, efforts must be made to mitigate degradation in order to maximize the potential impact of WBE on public health.
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