COVID-19 wastewater surveillance in rural communities: Comparison of lagoon and pumping station samples.
COVID-19 wastewater surveillance in rural communities: Comparison of lagoon and pumping station samples.
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DOI：
10.1016/j.scitotenv.2021.149618
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发表时间：
2021-12-20
期刊：
影响因子：
--
通讯作者：
Delatolla R
中科院分区：
文献类型：
--
作者：
D'Aoust PM;Towhid ST;Mercier É;Hegazy N;Tian X;Bhatnagar K;Zhang Z;Naughton CC;MacKenzie AE;Graber TE;Delatolla R
基于水的流行病学/废水监测在过去一年中一直是一个备受关注的话题，因为它在SARS-CoV-2监测中的应用可以追踪社区中COVID-19的流行情况。尽管迄今为止已在50多个国家实施了SARS-CoV-2监测，但这种监测的应用主要集中在相对富裕的城市和城郊社区。因此，在小型和农村社区实施可靠的废水监测以跟踪COVID-19和其他病原体或生物标志物的发病率方面存在知识差距。本研究考察了来自上游泵站和下游废水处理泻湖入口的废水样本的SARS-CoV-2病毒信号与加拿大一个小型农村社区的COVID-19发病率（以检测阳性百分比衡量）之间的关系。针对SARS-CoV-2的N1和N2基因的实时定量聚合酶链反应（RT-qPCR）表明，在5.5周的时间内从泵站收获的所有24小时复合样品都具有强烈的病毒信号，而在同一时期从泻湖收获的所有样品24小时复合样品都低于定量限。从泻湖收获的样品的RNA浓度和完整性都较低，更可变的比从上游泵站收集的样品在同一天，表明较高的SARS-CoV-2 RNA的泻湖上游的整体稳定性。此外，废水中PMMoV信号的测量允许将粪便物质含量的SARS-CoV-2病毒信号标准化，从而允许以高水平的粒度检测社区流行率的实际变化。因此，在有下水道的小型和农村社区或经营废水泻湖的低收入地区，用于废水监测的样本应从泵站或下水道收集，而不是从泻湖收集。
Wastewater-based epidemiology/wastewater surveillance has been a topic of significant interest over the last year due to its application in SARS-CoV-2 surveillance to track prevalence of COVID-19 in communities. Although SARS-CoV-2 surveillance has been applied in more than 50 countries to date, the application of this surveillance has been largely focused on relatively affluent urban and peri-urban communities. As such, there is a knowledge gap regarding the implementation of reliable wastewater surveillance in small and rural communities for the purpose of tracking rates of incidence of COVID-19 and other pathogens or biomarkers. This study examines the relationships existing between SARS-CoV-2 viral signal from wastewater samples harvested from an upstream pumping station and from an access port at a downstream wastewater treatment lagoon with the community's COVID-19 rate of incidence (measured as percent test positivity) in a small, rural community in Canada. Real-time quantitative polymerase chain reaction (RT-qPCR) targeting the N1 and N2 genes of SARS-CoV-2 demonstrate that all 24-h composite samples harvested from the pumping station over a period of 5.5 weeks had strong viral signal, while all samples 24-h composite samples harvested from the lagoon over the same period were below the limit of quantification. RNA concentrations and integrity of samples harvested from the lagoon were both lower and more variable than from samples from the upstream pumping station collected on the same date, indicating a higher overall stability of SARS-CoV-2 RNA upstream of the lagoon. Additionally, measurements of PMMoV signal in wastewater allowed normalizing SARS-CoV-2 viral signal for fecal matter content, permitting the detection of actual changes in community prevalence with a high level of granularity. As a result, in sewered small and rural communities or low-income regions operating wastewater lagoons, samples for wastewater surveillance should be harvested from pumping stations or the sewershed as opposed to lagoons.
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DOI：
10.1016/j.scitotenv.2020.144105
发表时间：
2021-02-20
期刊：
The Science of the total environment
影响因子：
--
作者：
Barril PA;Pianciola LA;Mazzeo M;Ousset MJ;Jaureguiberry MV;Alessandrello M;Sánchez G;Oteiza JM
通讯作者：
Oteiza JM
影响因子：
2
作者：
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通讯作者：
Delatolla, Robert
DOI：
10.1061/(asce)wr.1943-5452.0000552
发表时间：
2016-01-01
影响因子：
3.1
作者：
Haider, Husnain;Sadiq, Rehan;Tesfamariam, Solomon
通讯作者：
Tesfamariam, Solomon
影响因子：
11.4
作者：
Graham, Katherine E.;Loeb, Stephanie K.;Boehm, Alexandria B.
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影响因子：
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作者：
Kumar M;Joshi M;Patel AK;Joshi CG
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