Mildew Locus O facilitates colonization by arbuscular mycorrhiza in angiosperms

Mildew Locus O facilitates colonization by arbuscular mycorrhiza in angiosperms
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霉位点O促进被子植物中丛枝菌根的定植

DOI:
10.1101/851840
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发表时间:
2019
期刊:
--
影响因子:
--
通讯作者:
Jacott C
Jacott C
中科院分区:
--
文献类型:
--
作者:
Jacott C

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众所周知,大麦抗霉位点O(MLO)的丧失赋予了大麦对白粉病(Blumeria graminis)的持久和强大的抗性,白粉病是一种生物营养真菌叶片病原体。基于mloin在菌根中表达的增加以及它在MLO家族的一个分支中的存在,我们研究了MLO在丛枝菌根相互作用中的潜在作用。利用大麦、小麦和苜蓿的突变体,我们证明了MLO在丛枝菌根真菌不规则食根菌(girhizophagus irregularis)定植中的作用。在大麦、小麦和苜蓿的突变体中,早期菌根定植减少,这伴随着许多对丛枝菌根真菌在细胞内调节所需的关键基因的表达明显减少。这些发现表明,MLO在被子植物中的主要作用是与有益真菌建立共生关系,白粉病已经利用了这一作用。
Loss of barleyMildew Resistance Locus O(MLO) is known to confer durable and robust resistance to powdery mildew (Blumeria graminis), a biotrophic fungal leaf pathogen. Based on the increased expression ofMLOin mycorrhizal roots and its presence in a clade of the MLO family that is specific to mycorrhizal-host species, we investigated the potential role of MLO in arbuscular mycorrhizal interactions.Using mutants from barley, wheat, andMedicago truncatula, we demonstrate a role for MLO in colonization by the arbuscular mycorrhizal fungiRhizophagus irregularis.Early mycorrhizal colonization was reduced inmlomutants of barley, wheat andMedicago truncatula, and this was accompanied by a pronounced decrease in the expression of many of the key genes required for intracellular accommodation of arbuscular mycorrhizal fungi.These findings suggest that the primary role of MLO in angiosperms is in the establishment of symbiotic associations with beneficial fungi, which has been appropriated by powdery mildew.
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