Expression of mRNA electroporated into plant and animal cells.
Expression of mRNA electroporated into plant and animal cells.
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mRNA 的表达电穿孔到植物和动物细胞中。
DOI：
10.1093/nar/15.14.5823
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发表时间：
1987
影响因子：
14.9
通讯作者：
Walbot,V
中科院分区：
文献类型：
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作者：
Callis,J;Fromm,M;Walbot,V
本文介绍了将RNA分子导入植物原生质体和动物细胞的一般方法。该技术利用高场强的电脉冲在生物膜中形成孔的能力。含有细菌酶氯霉素乙酰转移酶（CAT）的编码区的RNA分子被用作模型系统。由于引入的RNA的体内翻译而导致的CAT活性的存在完全依赖于5′帽的存在，并且通过3′端的poly A尾的存在而大大增加。将RNA引入真核细胞中具有广泛的适用性，既可作为不依赖于转录活性的核酸摄取到细胞中的测定，也可作为研究真核mRNA翻译的工具。
A general method to introduce RNA molecules into plant protoplasts and animal cells is described. This technique utilizes the ability of electric pulses of high field strength to form pores in biomembranes. RNA molecules containing the coding region for the bacterial enzyme chloramphenicol acetyltransferase (CAT) were used as a model system. The presence of CAT activity as a result of the in vivo translation of the introduced RNA is entirely dependent on the presence of a 5′ cap and greatly increased by the presence of a poly A tail at the 3′ end. The introduction of RNA into eukaiyotic cells has broad applicability both as an assay for the uptake of nucleic acids into cells independent of transcriptional activity and as a tool to study eukaryotic mRNA translation.
R.G.Duggleby：“酶学方法”
DOI：
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发表时间：
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影响因子：
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电场效应的微生物学意义 VII．
影响因子：
3.1
作者：
N. Shivarova;W. Förster;H. Jacob;R. Grigorova
通讯作者：
R. Grigorova