Optimizing perfusion-decellularization methods of porcine livers for clinical-scale whole-organ bioengineering.
Optimizing perfusion-decellularization methods of porcine livers for clinical-scale whole-organ bioengineering.
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DOI：
10.1155/2015/785474
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发表时间：
2015
影响因子：
--
通讯作者：
Bu H
中科院分区：
文献类型：
--
作者：
Wu Q;Bao J;Zhou YJ;Wang YJ;Du ZG;Shi YJ;Li L;Bu H
Aim. To refine the decellularization protocol of whole porcine liver, which holds great promise for liver tissue engineering. Methods. Three decellularization methods for porcine livers (1% sodium dodecyl sulfate (SDS), 1% Triton X-100 + 1% sodium dodecyl sulfate, and 1% sodium deoxycholate + 1% sodium dodecyl sulfate) were studied. The obtained liver scaffolds were processed for histology, residual cellular content analysis, and extracellular matrix (ECM) components evaluation to investigate decellularization efficiency and ECM preservation. Rat primary hepatocytes were seeded into three kinds of scaffold to detect the biocompatibility. Results. The whole liver decellularization was successfully achieved following all three kinds of treatment. SDS combined with Triton had a high efficacy of cellular removal and caused minimal disruption of essential ECM components; it was also the most biocompatible procedure for primary hepatocytes. Conclusion. We have refined a novel, standardized, time-efficient, and reproducible protocol for the decellularization of whole liver which can be further adapted to liver tissue engineering.
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影响因子：
2.7
作者：
Rosario, Derek J.;Reilly, Gwendolen C.;MacNeil, Sheila
通讯作者：
MacNeil, Sheila
DOI：
10.1126/science.1189345
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
通讯作者：
Niklason LE
影响因子：
23.4
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通讯作者：
Sievert, Karl-Dietrich
影响因子：
3.3
作者：
Bao J;Fisher JE;Lillegard JB;Wang W;Amiot B;Yu Y;Dietz AB;Nahmias Y;Nyberg SL
通讯作者：
Nyberg SL
影响因子：
2.2
作者：
Barakat, Omar;Abbasi, Shahrzad;Gauthier, Polly K.
通讯作者：
Gauthier, Polly K.