Tissue-engineered lungs for in vivo implantation.
Tissue-engineered lungs for in vivo implantation.
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DOI：
10.1126/science.1189345
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发表时间：
2010-07-30
期刊：
影响因子：
--
通讯作者：
Niklason LE
中科院分区：
文献类型：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
由于成人肺组织再生能力有限，肺移植是严重受损肺的主要治疗方法。为了探索肺组织是否可以在体外再生，我们使用了一种程序来处理成年大鼠的肺，这种程序去除了细胞成分，但留下了细胞外基质的支架，保留了呼吸道和血管系统的分级分支结构。然后，我们使用生物反应器在无细胞肺基质上培养肺上皮和血管内皮细胞。种植的内皮细胞在基质中表现出明显的层次化组织，并且种植的内皮细胞有效地重新填充了血管间室。在体外，工程肺的力学特性与天然肺组织相似，当植入大鼠体内的短时间间隔(45-120分钟)时，工程肺参与气体交换。这些结果表明，肺基质再生是一种可行的肺再生策略，虽然这只是朝着在体外生成全功能肺的最终目标迈出的第一步。
Because adult lung tissue has limited regeneration capacity, lung transplantation is the primary therapy for severely damaged lungs. To explore whether lung tissue can be regenerated in vitro, we treated lungs from adult rats using a procedure that removes cellular components but leaves behind a scaffold of extracellular matrix that retains the hierarchical branching structures of airways and vasculature. We then used a bioreactor to culture pulmonary epithelium and vascular endothelium on the acellular lung matrix. The seeded epithelium displayed remarkable hierarchical organization within the matrix, and the seeded endothelial cells efficiently repopulated the vascular compartment. In vitro, the mechanical characteristics of the engineered lungs were similar to those of native lung tissue, and when implanted into rats in vivo for short time intervals (45 to 120 minutes) the engineered lungs participated in gas exchange. Although representing only an initial step toward the ultimate goal of generating fully functional lungs in vitro, these results suggest that repopulation of lung matrix is a viable strategy for lung regeneration.
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