Elevated epidermal growth factor receptor gene copy number and expression in a squamous carcinoma cell line.
Elevated epidermal growth factor receptor gene copy number and expression in a squamous carcinoma cell line.
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鳞状癌细胞系中表皮生长因子受体基因拷贝数和表达升高。
DOI：
10.1172/jci111770
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
I. Pastan
中科院分区：
文献类型：
--
作者：
G. Merlino;Y. H. Xu;N. Richert;A. Clark;S. Ishii;S. Banks;I. Pastan
已知人表皮生长因子（EGF）受体与禽成红细胞增多症病毒的v-er B B癌基因蛋白同源。EGF受体基因在A431表皮样癌细胞中的过表达是由于基因扩增。在这项研究中，各种鳞状细胞癌进行了检查，SCC-15，含有高水平的EGF受体，通过免疫沉淀法确定通过EGF受体特异性多克隆抗体。使用克隆的EGF受体互补DNA作为探针，发现EGF受体RNA的水平在SCC-15中相对于正常培养的角质形成细胞升高了4倍。当使用相同的探针在基因组DNA印迹上鉴定EGF受体基因片段时，显示SCC-15细胞系具有扩增4至5倍的EGF受体基因拷贝数。基因扩增导致几种癌中EGF受体水平的增强，并可能是这些细胞中转化表型出现的原因。
The human epidermal growth factor (EGF) receptor is known to be homologous to the v-erb B oncogene protein of the avian erythroblastosis virus. Overexpression of the EGF receptor gene in A431 epidermoid carcinoma cells is due to gene amplification. In this study, a variety of squamous cell carcinomas were examined and one, SCC-15, contained high levels of the EGF receptor as determined by immunoprecipitation via an EGF receptor-specific polyclonal antibody. Using a cloned EGF receptor complementary DNA as a probe, the level of EGF receptor RNA was found to be elevated four-fold in SCC-15 relative to normal cultured keratinocytes. When the same probe was used to identify EGF receptor gene fragments on a genomic DNA blot, the SCC-15 cell line was shown to possess an EGF receptor gene copy number amplified four to five times. Gene amplification results in the enhancement in the level of the EGF receptor in several carcinomas and could be responsible for the appearance of the transformed phenotype in these cells.
影响因子：
11.2
作者：
J. Rheinwald;M. Beckett
通讯作者：
J. Rheinwald;M. Beckett